Proline and glycinebetaine ameliorated NaCl stress via scavenging of hydrogen peroxide and methylglyoxal but not superoxide or nitric oxide in tobacco cultured cells

Mst Nasrin Akhter Banu, Md Anamul Hoque, Megumi Watanabe-Sugimoto, Mohammad Muzahidul Islam, Misugi Uraji, Ken Matsuoka, Yoshimasa Nakamura, Yoshiyuki Murata

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Efficient detoxification of the reactive oxygen species, nitric oxide (NO) and methylglyoxal (MG), provides protection against NaCl-induced damage in plants. To elucidate the protective mechanisms of proline and glycinebetaine (betaine) against NaCl stress, intracellular levels of hydrogen peroxide (H 2O2), superoxide (O2-), NO, and MG were investigated in tobacco Bright Yellow-2 cells. The Levels of H 2O2, O2-, NO and MG were higher in the short-term and long-term NaClstressed cells than in the non-stressed cells, whereas the O2- level was higher in the long-term stressed cells. Exogenous proline and betaine decreased the H2O2 level in both the short-term and the long-term NaCl-stressed cells and the MG level in the long-term NaCl-stressed cells, but did not change the O 2- or NO levels. Under salt stress, both proline and betaine increased the transcription levels of glutathione peroxidase, which can contribute to the reduction of H2O2. In conclusion, proline and betaine mitigated salt stress via reduction of H2O 2 accumulation during short-term incubation and via reduction of the accumulation of H2O2 and MG during long-term incubation.

Original languageEnglish
Pages (from-to)2043-2049
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number10
DOIs
Publication statusPublished - 2010

Fingerprint

Pyruvaldehyde
Tobacco
Scavenging
Proline
Superoxides
Hydrogen Peroxide
Cultured Cells
Nitric Oxide
Cells
Salts
Detoxification
Transcription
Glutathione Peroxidase
Reactive Oxygen Species
stachydrine

Keywords

  • Antioxidant defense
  • Glycinebetaine
  • Methylglyoxal
  • Proline
  • Salt stress

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Proline and glycinebetaine ameliorated NaCl stress via scavenging of hydrogen peroxide and methylglyoxal but not superoxide or nitric oxide in tobacco cultured cells. / Akhter Banu, Mst Nasrin; Hoque, Md Anamul; Watanabe-Sugimoto, Megumi; Islam, Mohammad Muzahidul; Uraji, Misugi; Matsuoka, Ken; Nakamura, Yoshimasa; Murata, Yoshiyuki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 10, 2010, p. 2043-2049.

Research output: Contribution to journalArticle

Akhter Banu, Mst Nasrin ; Hoque, Md Anamul ; Watanabe-Sugimoto, Megumi ; Islam, Mohammad Muzahidul ; Uraji, Misugi ; Matsuoka, Ken ; Nakamura, Yoshimasa ; Murata, Yoshiyuki. / Proline and glycinebetaine ameliorated NaCl stress via scavenging of hydrogen peroxide and methylglyoxal but not superoxide or nitric oxide in tobacco cultured cells. In: Bioscience, Biotechnology and Biochemistry. 2010 ; Vol. 74, No. 10. pp. 2043-2049.
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