Proliferation of smooth muscle cells stimulated by porphyromonas gingivalis is inhibited by apple polyphenol

Hiroaki Inaba, Motoyuki Tagashira, Tomomasa Kanda, Atsuo Amano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Porphyromonas gingivalis (Pg) is thought to be involved in the progression of occlusive arterial lesions, whereas vascular smooth muscle cell (SMC) proliferation is considered to be involved in occlusive arterial disease. We previously showed that bacteremia caused by Pg infection induced proliferation of mouse aortic SMCs. Furthermore, human SMCs stimulated with human plasma incubated with Pg showed a marked transformation from the contractile to proliferative phenotype. In the present study, we examine the involvement of Pg gingipains and fimbriae in induction of the SMC transformation and proliferation, and effective inhibitors. Methods: Pg strains including gingipain- and fimbria-null mutants were incubated in human plasma, after which the bacteria were removed and the supernatants were added to cultured SMCs. To evaluate the effects of inhibitors, Pg organisms were incubated in plasma in the presence of apple polyphenol (AP), epigallocatechin gallate, KYT-1 (Arg-gingipain inhibitor), and KYT-36 (Lys-gingipain inhibitor). Results: Plasma supernatants from wild-type and fimbriamutant cultures markedly stimulated cellular proliferation, whereas those containing gingipain-null mutants showed negligible effects. SMC proliferation was also induced by plasma treated with trypsin. Furthermore, plasma supernatants cultured in the presence of KYT-1/KYT-36 and AP showed significant inhibitory effects on SMC proliferation, whereas cultures with epigallocatechin gallate did not. Conclusion: Our results suggest that Pg gingipains are involved in the induction of SMC transformation and proliferation, whereas this was inhibited by AP.

Original languageEnglish
Pages (from-to)1616-1622
Number of pages7
JournalJournal of Periodontology
Volume82
Issue number11
DOIs
Publication statusPublished - Nov 2011
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Malus
Polyphenols
Smooth Muscle Myocytes
Cell Proliferation
Arterial Occlusive Diseases
Bacteremia
Vascular Smooth Muscle
Trypsin
Porphyromonas gingivalis argingipain
Bacteria
Phenotype
Infection

Keywords

  • Epigallocatechin gallate
  • Polyphenol
  • Porphyromonas gingivalis
  • Protease

ASJC Scopus subject areas

  • Periodontics

Cite this

Proliferation of smooth muscle cells stimulated by porphyromonas gingivalis is inhibited by apple polyphenol. / Inaba, Hiroaki; Tagashira, Motoyuki; Kanda, Tomomasa; Amano, Atsuo.

In: Journal of Periodontology, Vol. 82, No. 11, 11.2011, p. 1616-1622.

Research output: Contribution to journalArticle

Inaba, Hiroaki ; Tagashira, Motoyuki ; Kanda, Tomomasa ; Amano, Atsuo. / Proliferation of smooth muscle cells stimulated by porphyromonas gingivalis is inhibited by apple polyphenol. In: Journal of Periodontology. 2011 ; Vol. 82, No. 11. pp. 1616-1622.
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