Proliferation and insulin secretion function of mouse insulinoma cells encapsulated in alginate/sol-gel synthesized aminopropyl-silicate/alginate microcapsule

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Alginate/aminopropyl-silicate/alginate microcapsules, ca. 15 μm in membrane thickness and ca. 500 μm in diameter, were prepared via sol-gel process. The aminopropyl-silicate membrane was derived from two silicone alkoxide precursors, tetramethoxysilane and 3-aminopropyl-trimethoxysilane on Ca-alginate micro gel beads. Pancreatic β-cell line (MIN6) cells were encapsulated in the microcapsule. The encapsulated MIN6 cells proliferated and formed spheroidal tissues in vitro. The diameter of the MIN6 spheroids increased to approximately 250 μm with an increase in the incubation period until the day 35. Storeptozotocin-induced diabetic mice became normoglycemia after implantation of the MIN6-enclosing microcapsules. The normoglycemic state remained until the retrieval of the implanted microcapsules for 1 month. These results indicate that the potential use of the alginate/aminopropyl-silicate/alginate microcapsule as a vehicle for a genetically engineered cell-enclosing therapeutic material delivery system.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalJournal of Sol-Gel Science and Technology
Volume28
Issue number2
DOIs
Publication statusPublished - Sep 1 2003
Externally publishedYes

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Keywords

  • Aminopropyl-silicate
  • Cell encapsulation
  • MIN6
  • Microcapsule
  • Sol-gel process

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Biomaterials
  • Condensed Matter Physics
  • Materials Chemistry

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