Prolactin, Growth Hormone, and Luteinizing Hormone-β Subunit Gene Expression in the Cephalic and Caudal Lobes of the Anterior Pituitary Gland during Embryogenesis and Different Reproductive Stages in the Chicken

Norio Kansaku, Kiyoshi Shimada, Osamu Terada, Noboru Saito

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Messenger RNA (mRNA) levels of prolactin (PRL), growth hormone (GH), and luteinizing hormone (LH)-β subunit in the cephalic and caudal lobes of the anterior pituitary gland of the chicken during embryonic development and different reproductive stages were determined by Northern blot analysis and are related to hormone levels in plasma and each lobe. Steady-state levels of 1.4-kb transcript of PRL of the cephalic and caudal lobes increased on Days 18 and 20 of incubation, respectively. The levels were greater in the cephalic lobe than in the caudal lobe on Days 18 and 20 of incubation. Levels of PRL in each lobe show similar changes to those of mRNA levels. GH mRNA levels of both lobes started to increase on Day 16 of incubation but the levels were higher in the caudal lobe than in the cephalic lobe on Days 16-20 of incubation. Levels of GH in the cephalic lobe increased discontinuously, while those in the caudal lobe increased continuously on days of incubation. LH-β mRNA was not detected in embryos. Levels of PRL mRNA increased approximately 18-, 20-, and 3-fold in hens at 1 and 3 weeks of egg incubation and brooding stages in the hen, relative to prelaying, respectively. These levels were higher in the cephalic lobe than in the caudal lobe at all stages except for prelaying stages. Levels of PRL in each lobe displayed essentially similar changes to the mRNA levels. GH mRNA levels did not significantly vary during the cycle but were higher in the caudal lobe than in the cephalic lobe. There was no difference in LH-β mRNA levels between the two lobes but the levels were higher at prelaying and laying stages than at incubation and brooding stages. The same changes in LH concentrations in plasma as those of LH-β mRNA were found at the stages. This study shows regional differences between PRL and GH mRNA levels in the anterior pituitary gland in relation to embryogenesis and different reproductive stages.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume96
Issue number2
DOIs
Publication statusPublished - Nov 1994
Externally publishedYes

Fingerprint

anterior pituitary
Anterior Pituitary Gland
luteinizing hormone
messenger RNA
Luteinizing Hormone
prolactin
Prolactin
somatotropin
Growth Hormone
Embryonic Development
Chickens
embryogenesis
Head
chickens
Gene Expression
troglitazone
gene expression
Messenger RNA
hens
egg incubation

ASJC Scopus subject areas

  • Endocrinology

Cite this

@article{d866f6e40f254bada1b836db477c1960,
title = "Prolactin, Growth Hormone, and Luteinizing Hormone-β Subunit Gene Expression in the Cephalic and Caudal Lobes of the Anterior Pituitary Gland during Embryogenesis and Different Reproductive Stages in the Chicken",
abstract = "Messenger RNA (mRNA) levels of prolactin (PRL), growth hormone (GH), and luteinizing hormone (LH)-β subunit in the cephalic and caudal lobes of the anterior pituitary gland of the chicken during embryonic development and different reproductive stages were determined by Northern blot analysis and are related to hormone levels in plasma and each lobe. Steady-state levels of 1.4-kb transcript of PRL of the cephalic and caudal lobes increased on Days 18 and 20 of incubation, respectively. The levels were greater in the cephalic lobe than in the caudal lobe on Days 18 and 20 of incubation. Levels of PRL in each lobe show similar changes to those of mRNA levels. GH mRNA levels of both lobes started to increase on Day 16 of incubation but the levels were higher in the caudal lobe than in the cephalic lobe on Days 16-20 of incubation. Levels of GH in the cephalic lobe increased discontinuously, while those in the caudal lobe increased continuously on days of incubation. LH-β mRNA was not detected in embryos. Levels of PRL mRNA increased approximately 18-, 20-, and 3-fold in hens at 1 and 3 weeks of egg incubation and brooding stages in the hen, relative to prelaying, respectively. These levels were higher in the cephalic lobe than in the caudal lobe at all stages except for prelaying stages. Levels of PRL in each lobe displayed essentially similar changes to the mRNA levels. GH mRNA levels did not significantly vary during the cycle but were higher in the caudal lobe than in the cephalic lobe. There was no difference in LH-β mRNA levels between the two lobes but the levels were higher at prelaying and laying stages than at incubation and brooding stages. The same changes in LH concentrations in plasma as those of LH-β mRNA were found at the stages. This study shows regional differences between PRL and GH mRNA levels in the anterior pituitary gland in relation to embryogenesis and different reproductive stages.",
author = "Norio Kansaku and Kiyoshi Shimada and Osamu Terada and Noboru Saito",
year = "1994",
month = "11",
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journal = "General and Comparative Endocrinology",
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T1 - Prolactin, Growth Hormone, and Luteinizing Hormone-β Subunit Gene Expression in the Cephalic and Caudal Lobes of the Anterior Pituitary Gland during Embryogenesis and Different Reproductive Stages in the Chicken

AU - Kansaku, Norio

AU - Shimada, Kiyoshi

AU - Terada, Osamu

AU - Saito, Noboru

PY - 1994/11

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N2 - Messenger RNA (mRNA) levels of prolactin (PRL), growth hormone (GH), and luteinizing hormone (LH)-β subunit in the cephalic and caudal lobes of the anterior pituitary gland of the chicken during embryonic development and different reproductive stages were determined by Northern blot analysis and are related to hormone levels in plasma and each lobe. Steady-state levels of 1.4-kb transcript of PRL of the cephalic and caudal lobes increased on Days 18 and 20 of incubation, respectively. The levels were greater in the cephalic lobe than in the caudal lobe on Days 18 and 20 of incubation. Levels of PRL in each lobe show similar changes to those of mRNA levels. GH mRNA levels of both lobes started to increase on Day 16 of incubation but the levels were higher in the caudal lobe than in the cephalic lobe on Days 16-20 of incubation. Levels of GH in the cephalic lobe increased discontinuously, while those in the caudal lobe increased continuously on days of incubation. LH-β mRNA was not detected in embryos. Levels of PRL mRNA increased approximately 18-, 20-, and 3-fold in hens at 1 and 3 weeks of egg incubation and brooding stages in the hen, relative to prelaying, respectively. These levels were higher in the cephalic lobe than in the caudal lobe at all stages except for prelaying stages. Levels of PRL in each lobe displayed essentially similar changes to the mRNA levels. GH mRNA levels did not significantly vary during the cycle but were higher in the caudal lobe than in the cephalic lobe. There was no difference in LH-β mRNA levels between the two lobes but the levels were higher at prelaying and laying stages than at incubation and brooding stages. The same changes in LH concentrations in plasma as those of LH-β mRNA were found at the stages. This study shows regional differences between PRL and GH mRNA levels in the anterior pituitary gland in relation to embryogenesis and different reproductive stages.

AB - Messenger RNA (mRNA) levels of prolactin (PRL), growth hormone (GH), and luteinizing hormone (LH)-β subunit in the cephalic and caudal lobes of the anterior pituitary gland of the chicken during embryonic development and different reproductive stages were determined by Northern blot analysis and are related to hormone levels in plasma and each lobe. Steady-state levels of 1.4-kb transcript of PRL of the cephalic and caudal lobes increased on Days 18 and 20 of incubation, respectively. The levels were greater in the cephalic lobe than in the caudal lobe on Days 18 and 20 of incubation. Levels of PRL in each lobe show similar changes to those of mRNA levels. GH mRNA levels of both lobes started to increase on Day 16 of incubation but the levels were higher in the caudal lobe than in the cephalic lobe on Days 16-20 of incubation. Levels of GH in the cephalic lobe increased discontinuously, while those in the caudal lobe increased continuously on days of incubation. LH-β mRNA was not detected in embryos. Levels of PRL mRNA increased approximately 18-, 20-, and 3-fold in hens at 1 and 3 weeks of egg incubation and brooding stages in the hen, relative to prelaying, respectively. These levels were higher in the cephalic lobe than in the caudal lobe at all stages except for prelaying stages. Levels of PRL in each lobe displayed essentially similar changes to the mRNA levels. GH mRNA levels did not significantly vary during the cycle but were higher in the caudal lobe than in the cephalic lobe. There was no difference in LH-β mRNA levels between the two lobes but the levels were higher at prelaying and laying stages than at incubation and brooding stages. The same changes in LH concentrations in plasma as those of LH-β mRNA were found at the stages. This study shows regional differences between PRL and GH mRNA levels in the anterior pituitary gland in relation to embryogenesis and different reproductive stages.

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