Proinflammatory gene expression in mouse ST2 cell line in response to infection by Porphyromonas gingivalis

Takashi Ohno, Nobuo Okahashi, Shinji Kawai, Takahiro Kato, Hiroaki Inaba, Yasuko Shibata, Ichijiro Morisaki, Yoshimitsu Abiko, Atsuo Amano

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Porphyromonas gingivalis is a predominant periodontal pathogen, whose infection causes inflammatory responses in periodontal tissue and alveolar bone resorption. Various virulence factors of this pathogen modulate host innate immune responses. It has been reported that gingipains degrade a wide variety of host cell proteins, and fimbriae are involved in bacterial adhesion to and invasion of host cells. In the present study, we profiled ST2 stromal cell gene expression following infection with the viable P. gingivalis strain ATCC33277 as well as with its gingipain- and fimbriae-deficient mutants, using microarray technology and quantitative real-time polymerase chain reaction. Using a mouse array of about 20,000 genes, we found that infection with the wild strain elicited a significant upregulation (greater than 2-fold) of expression of about 360 genes in ST2 cells, which included the chemokines CCL2, CCL5, and CXCL10, and other proinflammatory proteins such as interleukin-6 (IL-6) and matrix metalloproteinase-13 (MMP-13). Further, infection with the gingipain-deficient mutant elicited a reduced expression of the CXCL10, IL-6 and MMP-13 genes, suggesting that gingipains play an important role in inducing the expression of those genes following P. gingivalis infection. On the other hand, the pattern of global gene expression induced by the fimbriae-deficient mutant was similar to that by the wild strain. These results suggest that P. gingivalis infection induces gene expression of a wide variety of proinflammatory proteins in stromal cells/osteoblasts, and gingipains may be involved in inducing several of the proinflammatory factors.

Original languageEnglish
Pages (from-to)1025-1034
Number of pages10
JournalMicrobes and Infection
Volume8
Issue number4
DOIs
Publication statusPublished - Apr 2006
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Gene Expression
Cell Line
Infection
Matrix Metalloproteinase 13
Stromal Cells
Interleukin-6
Fimbriae Proteins
Chemokine CXCL10
Genes
Bacterial Adhesion
Alveolar Bone Loss
Chemokine CCL5
Chemokine CCL2
Virulence Factors
Bone Resorption
Osteoblasts
Innate Immunity
Real-Time Polymerase Chain Reaction
Proteins

Keywords

  • Gene expression
  • Inflammation
  • Microarray
  • Osteoblast
  • Periodontitis
  • Porphyromonas gingivalis
  • Stromal cell

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Proinflammatory gene expression in mouse ST2 cell line in response to infection by Porphyromonas gingivalis. / Ohno, Takashi; Okahashi, Nobuo; Kawai, Shinji; Kato, Takahiro; Inaba, Hiroaki; Shibata, Yasuko; Morisaki, Ichijiro; Abiko, Yoshimitsu; Amano, Atsuo.

In: Microbes and Infection, Vol. 8, No. 4, 04.2006, p. 1025-1034.

Research output: Contribution to journalArticle

Ohno, T, Okahashi, N, Kawai, S, Kato, T, Inaba, H, Shibata, Y, Morisaki, I, Abiko, Y & Amano, A 2006, 'Proinflammatory gene expression in mouse ST2 cell line in response to infection by Porphyromonas gingivalis', Microbes and Infection, vol. 8, no. 4, pp. 1025-1034. https://doi.org/10.1016/j.micinf.2005.10.021
Ohno, Takashi ; Okahashi, Nobuo ; Kawai, Shinji ; Kato, Takahiro ; Inaba, Hiroaki ; Shibata, Yasuko ; Morisaki, Ichijiro ; Abiko, Yoshimitsu ; Amano, Atsuo. / Proinflammatory gene expression in mouse ST2 cell line in response to infection by Porphyromonas gingivalis. In: Microbes and Infection. 2006 ; Vol. 8, No. 4. pp. 1025-1034.
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AU - Inaba, Hiroaki

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AU - Morisaki, Ichijiro

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AU - Amano, Atsuo

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