Programmed cell death protein 1 and programmed death-ligand 1 are expressed on the surface of some small-cell lung cancer lines

Hiromichi Yamane, Hideko Isozaki, Masami Takeyama, Nobuaki Ochi, Kenichiro Kudo, Yoshihiro Honda, Tomoko Yamagishi, Toshio Kubo, Katsuyuki Kiura, Nagio Takigawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Introduction: Programmed cell death protein 1 (PD-1) and programmed death-ligand 1 (PD-L1) play a major role in suppressing the immune system during the formation of the PD-1/PD-L1 pathway, which transmits an inhibitory signal to reduce T cell activity. PD-L1 is often expressed in various malignant tumors. In contrast, PD-1 is generally observed in activated lymphocytes and myeloid-derived dendritic cells. Of the malignant cells, only Jurkat cells under special conditions and angioimmunoblastic T-cell lymphoma tissue cells express PD-1 on their surface. Methods: To clarify whether the PD-1/PD-L1 pathway participates in the immunotolerance of small-cell lung cancer (SCLC) cells, we examined the expressions of PD-1 and PD-L1 on the cell surface of SCLC cell lines using flow cytometry and reverse transcription polymerase chain reaction. Results: Among the four SCLC cell lines examined, only SBC-3 expressed both PD-1 and PD-L1. Conclusions: We demonstrated that both PD-1 and PD-L1 molecules were co-expressed on the surface of SCLC cells. Although the biological implications of this remain unclear, we speculate that PD-1 and its ligand on the SCLC cells may participate in the growth inhibition of tumor cells as reported in cytotoxic T cells.

Original languageEnglish
Pages (from-to)1553-1557
Number of pages5
JournalAmerican Journal of Cancer Research
Volume5
Issue number4
Publication statusPublished - 2015

Fingerprint

Programmed Cell Death 1 Receptor
Small Cell Lung Carcinoma
Ligands
CD274 Antigen
T-Lymphocytes
Cell Line
Jurkat Cells
T-Cell Lymphoma
Dendritic Cells
Reverse Transcription
Immune System
Neoplasms
Flow Cytometry
Lymphocytes
Polymerase Chain Reaction

Keywords

  • Cell line
  • Co-expression
  • PD-1
  • PD-l1
  • Small-cell lung cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Yamane, H., Isozaki, H., Takeyama, M., Ochi, N., Kudo, K., Honda, Y., ... Takigawa, N. (2015). Programmed cell death protein 1 and programmed death-ligand 1 are expressed on the surface of some small-cell lung cancer lines. American Journal of Cancer Research, 5(4), 1553-1557.

Programmed cell death protein 1 and programmed death-ligand 1 are expressed on the surface of some small-cell lung cancer lines. / Yamane, Hiromichi; Isozaki, Hideko; Takeyama, Masami; Ochi, Nobuaki; Kudo, Kenichiro; Honda, Yoshihiro; Yamagishi, Tomoko; Kubo, Toshio; Kiura, Katsuyuki; Takigawa, Nagio.

In: American Journal of Cancer Research, Vol. 5, No. 4, 2015, p. 1553-1557.

Research output: Contribution to journalArticle

Yamane, H, Isozaki, H, Takeyama, M, Ochi, N, Kudo, K, Honda, Y, Yamagishi, T, Kubo, T, Kiura, K & Takigawa, N 2015, 'Programmed cell death protein 1 and programmed death-ligand 1 are expressed on the surface of some small-cell lung cancer lines', American Journal of Cancer Research, vol. 5, no. 4, pp. 1553-1557.
Yamane, Hiromichi ; Isozaki, Hideko ; Takeyama, Masami ; Ochi, Nobuaki ; Kudo, Kenichiro ; Honda, Yoshihiro ; Yamagishi, Tomoko ; Kubo, Toshio ; Kiura, Katsuyuki ; Takigawa, Nagio. / Programmed cell death protein 1 and programmed death-ligand 1 are expressed on the surface of some small-cell lung cancer lines. In: American Journal of Cancer Research. 2015 ; Vol. 5, No. 4. pp. 1553-1557.
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AU - Yamagishi, Tomoko

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