Prognostic significance of Bcl-xL in human hepatocellular carcinoma

Jota Watanabe, Fumiki Kushihata, Kazuo Honda, Atsuro Sugita, Norihiko Tateishi, Katsumi Mominoki, Seiji Matsuda, Nobuaki Kobayashi

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Background Proliferation and apoptosis of liver cancer cells are closely related phenomena. We investigated the correlation between overexpression of Bcl-xL, an anti-apoptosis-related protein of the Bcl-2 family, and the clinical course of hepatocellular carcinoma (HCC). Methods Specimens from 7 HCC patients were used for Western blotting and immunoelectron microscopy tests. Samples from 33 HCC patients who had undergone hepatectomies were used for immunohistochemical staining. The degrees of expression of Bcl-xL and Ki-67, as an index of HCC mitosis severity, were each classified into 2 groups. Results With the use of Western blot analysis, enhanced immunoreactivity of Bcl-xL was found in cancerous specimens. Bcl-xL overexpression was found in cancer specimens in 21 of 33 patients (63.6%). The overall survival (P=.019) and disease-free survival (P=.030) rates of the group overexpressing Bcl-xL were definitely poorer. The Ki-67 higher labeling index LI > 10) group had a poorer survival rate (P=.016). There were significant correlations between Bcl-xL and overall survival and disease-free survival. Multivariate analyses revealed that Bcl-xL, tumor size, histologic portal invasion, and histologic metastatic foci were independent prognostic factors for overall survival and disease-free survival. Conclusions These results showed Bcl-xL in HCC specimens, suggesting that Bcl-xL was a significant prognostic factor for disease progression in human HCC.

Original languageEnglish
Pages (from-to)604-612
Number of pages9
JournalSurgery
Volume135
Issue number6
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Fingerprint

Hepatocellular Carcinoma
Disease-Free Survival
Survival
Western Blotting
Apoptosis
Immunoelectron Microscopy
Hepatectomy
Liver Neoplasms
Mitosis
Disease Progression
Neoplasms
Multivariate Analysis
Survival Rate
Staining and Labeling
Proteins

ASJC Scopus subject areas

  • Surgery

Cite this

Watanabe, J., Kushihata, F., Honda, K., Sugita, A., Tateishi, N., Mominoki, K., ... Kobayashi, N. (2004). Prognostic significance of Bcl-xL in human hepatocellular carcinoma. Surgery, 135(6), 604-612. https://doi.org/10.1016/j.surg.2003.11.015

Prognostic significance of Bcl-xL in human hepatocellular carcinoma. / Watanabe, Jota; Kushihata, Fumiki; Honda, Kazuo; Sugita, Atsuro; Tateishi, Norihiko; Mominoki, Katsumi; Matsuda, Seiji; Kobayashi, Nobuaki.

In: Surgery, Vol. 135, No. 6, 06.2004, p. 604-612.

Research output: Contribution to journalArticle

Watanabe, J, Kushihata, F, Honda, K, Sugita, A, Tateishi, N, Mominoki, K, Matsuda, S & Kobayashi, N 2004, 'Prognostic significance of Bcl-xL in human hepatocellular carcinoma', Surgery, vol. 135, no. 6, pp. 604-612. https://doi.org/10.1016/j.surg.2003.11.015
Watanabe J, Kushihata F, Honda K, Sugita A, Tateishi N, Mominoki K et al. Prognostic significance of Bcl-xL in human hepatocellular carcinoma. Surgery. 2004 Jun;135(6):604-612. https://doi.org/10.1016/j.surg.2003.11.015
Watanabe, Jota ; Kushihata, Fumiki ; Honda, Kazuo ; Sugita, Atsuro ; Tateishi, Norihiko ; Mominoki, Katsumi ; Matsuda, Seiji ; Kobayashi, Nobuaki. / Prognostic significance of Bcl-xL in human hepatocellular carcinoma. In: Surgery. 2004 ; Vol. 135, No. 6. pp. 604-612.
@article{d6cb90aa45774603a3cd33940ba830be,
title = "Prognostic significance of Bcl-xL in human hepatocellular carcinoma",
abstract = "Background Proliferation and apoptosis of liver cancer cells are closely related phenomena. We investigated the correlation between overexpression of Bcl-xL, an anti-apoptosis-related protein of the Bcl-2 family, and the clinical course of hepatocellular carcinoma (HCC). Methods Specimens from 7 HCC patients were used for Western blotting and immunoelectron microscopy tests. Samples from 33 HCC patients who had undergone hepatectomies were used for immunohistochemical staining. The degrees of expression of Bcl-xL and Ki-67, as an index of HCC mitosis severity, were each classified into 2 groups. Results With the use of Western blot analysis, enhanced immunoreactivity of Bcl-xL was found in cancerous specimens. Bcl-xL overexpression was found in cancer specimens in 21 of 33 patients (63.6{\%}). The overall survival (P=.019) and disease-free survival (P=.030) rates of the group overexpressing Bcl-xL were definitely poorer. The Ki-67 higher labeling index LI > 10) group had a poorer survival rate (P=.016). There were significant correlations between Bcl-xL and overall survival and disease-free survival. Multivariate analyses revealed that Bcl-xL, tumor size, histologic portal invasion, and histologic metastatic foci were independent prognostic factors for overall survival and disease-free survival. Conclusions These results showed Bcl-xL in HCC specimens, suggesting that Bcl-xL was a significant prognostic factor for disease progression in human HCC.",
author = "Jota Watanabe and Fumiki Kushihata and Kazuo Honda and Atsuro Sugita and Norihiko Tateishi and Katsumi Mominoki and Seiji Matsuda and Nobuaki Kobayashi",
year = "2004",
month = "6",
doi = "10.1016/j.surg.2003.11.015",
language = "English",
volume = "135",
pages = "604--612",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - Prognostic significance of Bcl-xL in human hepatocellular carcinoma

AU - Watanabe, Jota

AU - Kushihata, Fumiki

AU - Honda, Kazuo

AU - Sugita, Atsuro

AU - Tateishi, Norihiko

AU - Mominoki, Katsumi

AU - Matsuda, Seiji

AU - Kobayashi, Nobuaki

PY - 2004/6

Y1 - 2004/6

N2 - Background Proliferation and apoptosis of liver cancer cells are closely related phenomena. We investigated the correlation between overexpression of Bcl-xL, an anti-apoptosis-related protein of the Bcl-2 family, and the clinical course of hepatocellular carcinoma (HCC). Methods Specimens from 7 HCC patients were used for Western blotting and immunoelectron microscopy tests. Samples from 33 HCC patients who had undergone hepatectomies were used for immunohistochemical staining. The degrees of expression of Bcl-xL and Ki-67, as an index of HCC mitosis severity, were each classified into 2 groups. Results With the use of Western blot analysis, enhanced immunoreactivity of Bcl-xL was found in cancerous specimens. Bcl-xL overexpression was found in cancer specimens in 21 of 33 patients (63.6%). The overall survival (P=.019) and disease-free survival (P=.030) rates of the group overexpressing Bcl-xL were definitely poorer. The Ki-67 higher labeling index LI > 10) group had a poorer survival rate (P=.016). There were significant correlations between Bcl-xL and overall survival and disease-free survival. Multivariate analyses revealed that Bcl-xL, tumor size, histologic portal invasion, and histologic metastatic foci were independent prognostic factors for overall survival and disease-free survival. Conclusions These results showed Bcl-xL in HCC specimens, suggesting that Bcl-xL was a significant prognostic factor for disease progression in human HCC.

AB - Background Proliferation and apoptosis of liver cancer cells are closely related phenomena. We investigated the correlation between overexpression of Bcl-xL, an anti-apoptosis-related protein of the Bcl-2 family, and the clinical course of hepatocellular carcinoma (HCC). Methods Specimens from 7 HCC patients were used for Western blotting and immunoelectron microscopy tests. Samples from 33 HCC patients who had undergone hepatectomies were used for immunohistochemical staining. The degrees of expression of Bcl-xL and Ki-67, as an index of HCC mitosis severity, were each classified into 2 groups. Results With the use of Western blot analysis, enhanced immunoreactivity of Bcl-xL was found in cancerous specimens. Bcl-xL overexpression was found in cancer specimens in 21 of 33 patients (63.6%). The overall survival (P=.019) and disease-free survival (P=.030) rates of the group overexpressing Bcl-xL were definitely poorer. The Ki-67 higher labeling index LI > 10) group had a poorer survival rate (P=.016). There were significant correlations between Bcl-xL and overall survival and disease-free survival. Multivariate analyses revealed that Bcl-xL, tumor size, histologic portal invasion, and histologic metastatic foci were independent prognostic factors for overall survival and disease-free survival. Conclusions These results showed Bcl-xL in HCC specimens, suggesting that Bcl-xL was a significant prognostic factor for disease progression in human HCC.

UR - http://www.scopus.com/inward/record.url?scp=3042585770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042585770&partnerID=8YFLogxK

U2 - 10.1016/j.surg.2003.11.015

DO - 10.1016/j.surg.2003.11.015

M3 - Article

C2 - 15179366

AN - SCOPUS:3042585770

VL - 135

SP - 604

EP - 612

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 6

ER -