Abstract
Background Proliferation and apoptosis of liver cancer cells are closely related phenomena. We investigated the correlation between overexpression of Bcl-xL, an anti-apoptosis-related protein of the Bcl-2 family, and the clinical course of hepatocellular carcinoma (HCC). Methods Specimens from 7 HCC patients were used for Western blotting and immunoelectron microscopy tests. Samples from 33 HCC patients who had undergone hepatectomies were used for immunohistochemical staining. The degrees of expression of Bcl-xL and Ki-67, as an index of HCC mitosis severity, were each classified into 2 groups. Results With the use of Western blot analysis, enhanced immunoreactivity of Bcl-xL was found in cancerous specimens. Bcl-xL overexpression was found in cancer specimens in 21 of 33 patients (63.6%). The overall survival (P=.019) and disease-free survival (P=.030) rates of the group overexpressing Bcl-xL were definitely poorer. The Ki-67 higher labeling index LI > 10) group had a poorer survival rate (P=.016). There were significant correlations between Bcl-xL and overall survival and disease-free survival. Multivariate analyses revealed that Bcl-xL, tumor size, histologic portal invasion, and histologic metastatic foci were independent prognostic factors for overall survival and disease-free survival. Conclusions These results showed Bcl-xL in HCC specimens, suggesting that Bcl-xL was a significant prognostic factor for disease progression in human HCC.
| Original language | English |
|---|---|
| Pages (from-to) | 604-612 |
| Number of pages | 9 |
| Journal | Surgery |
| Volume | 135 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Surgery
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Prognostic significance of Bcl-xL in human hepatocellular carcinoma. / Watanabe, Jota; Kushihata, Fumiki; Honda, Kazuo; Sugita, Atsuro; Tateishi, Norihiko; Mominoki, Katsumi; Matsuda, Seiji; Kobayashi, Nobuaki.
In: Surgery, Vol. 135, No. 6, 06.2004, p. 604-612.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prognostic significance of Bcl-xL in human hepatocellular carcinoma
AU - Watanabe, Jota
AU - Kushihata, Fumiki
AU - Honda, Kazuo
AU - Sugita, Atsuro
AU - Tateishi, Norihiko
AU - Mominoki, Katsumi
AU - Matsuda, Seiji
AU - Kobayashi, Nobuaki
PY - 2004/6
Y1 - 2004/6
N2 - Background Proliferation and apoptosis of liver cancer cells are closely related phenomena. We investigated the correlation between overexpression of Bcl-xL, an anti-apoptosis-related protein of the Bcl-2 family, and the clinical course of hepatocellular carcinoma (HCC). Methods Specimens from 7 HCC patients were used for Western blotting and immunoelectron microscopy tests. Samples from 33 HCC patients who had undergone hepatectomies were used for immunohistochemical staining. The degrees of expression of Bcl-xL and Ki-67, as an index of HCC mitosis severity, were each classified into 2 groups. Results With the use of Western blot analysis, enhanced immunoreactivity of Bcl-xL was found in cancerous specimens. Bcl-xL overexpression was found in cancer specimens in 21 of 33 patients (63.6%). The overall survival (P=.019) and disease-free survival (P=.030) rates of the group overexpressing Bcl-xL were definitely poorer. The Ki-67 higher labeling index LI > 10) group had a poorer survival rate (P=.016). There were significant correlations between Bcl-xL and overall survival and disease-free survival. Multivariate analyses revealed that Bcl-xL, tumor size, histologic portal invasion, and histologic metastatic foci were independent prognostic factors for overall survival and disease-free survival. Conclusions These results showed Bcl-xL in HCC specimens, suggesting that Bcl-xL was a significant prognostic factor for disease progression in human HCC.
AB - Background Proliferation and apoptosis of liver cancer cells are closely related phenomena. We investigated the correlation between overexpression of Bcl-xL, an anti-apoptosis-related protein of the Bcl-2 family, and the clinical course of hepatocellular carcinoma (HCC). Methods Specimens from 7 HCC patients were used for Western blotting and immunoelectron microscopy tests. Samples from 33 HCC patients who had undergone hepatectomies were used for immunohistochemical staining. The degrees of expression of Bcl-xL and Ki-67, as an index of HCC mitosis severity, were each classified into 2 groups. Results With the use of Western blot analysis, enhanced immunoreactivity of Bcl-xL was found in cancerous specimens. Bcl-xL overexpression was found in cancer specimens in 21 of 33 patients (63.6%). The overall survival (P=.019) and disease-free survival (P=.030) rates of the group overexpressing Bcl-xL were definitely poorer. The Ki-67 higher labeling index LI > 10) group had a poorer survival rate (P=.016). There were significant correlations between Bcl-xL and overall survival and disease-free survival. Multivariate analyses revealed that Bcl-xL, tumor size, histologic portal invasion, and histologic metastatic foci were independent prognostic factors for overall survival and disease-free survival. Conclusions These results showed Bcl-xL in HCC specimens, suggesting that Bcl-xL was a significant prognostic factor for disease progression in human HCC.
UR - http://www.scopus.com/inward/record.url?scp=3042585770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042585770&partnerID=8YFLogxK
U2 - 10.1016/j.surg.2003.11.015
DO - 10.1016/j.surg.2003.11.015
M3 - Article
C2 - 15179366
AN - SCOPUS:3042585770
VL - 135
SP - 604
EP - 612
JO - Surgery
JF - Surgery
SN - 0039-6060
IS - 6
ER -