Progesterone stimulation by prostaglandin f involves the protein kinase c pathway in cultured bovine luteal cells

Kiyoshi Okuda, Yoshihisa Uenoyama, L. E.E. Kang Woo, Ryosuke Sakumoto, Dariusz Jan Skarzynski

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57 Citations (Scopus)


The intracellular mechanism that mediates the stimulatory effect of prostaglandin F (PGF) on progesterone secretion by bovine luteal cells is not well understood. In the present study, we investigated to test the hypothesis that PGF stimulates progesterone secretion through activation of protein kinase C (PKC) by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to varying concentrations of PGF (10-8, 10-7, 10-6 M) or luteinizing hormone (LH; 100 ng/ml) for 4, 6, or 16 h. Treatment of the cells with the highest dose of PGF (10-6 M) or LH resulted in increases in progesterone secretion for all three exposure times. Following down-regulation of PKC with a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (PMA; 10-6 M), the stimulatory effect of PGP was no longer evident, whereas the response to LH was unaffected. These results support the hypothesis that the luteotropic action of PGF is mediated by the activation of intracellular PKC.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalJournal of Reproduction and Development
Issue number1
Publication statusPublished - 1998


  • Corpus luteum
  • Cow
  • Progesterone
  • Prostaglandin f
  • Protein kinase c

ASJC Scopus subject areas

  • Animal Science and Zoology


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