Progesterone stimulation by LH involves the phospholipase-C pathway in bovine luteal cells

Ryo Nishimura, Masami Shibaya, Dariusz J. Skarzynski, Kiyoshi Okuda

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Luteinizing hormone (LH)-stimulated steroidogenesis in luteal cells is known to be mediated through the activation of cyclic AMP (cAMP)-dependent protein kinase, and to be also modulated by calcium-dependent mechanisms. In the present study, we tested the hypothesis that LH stimulates progesterone (P4) production in bovine luteal cells through activation of phospholipase (PL) C by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to a PLC inhibitor (U-73122; 10 μM) with or without LH (10 ng/ml) for 4 h. U-73122 blocked LH-stimulated P4 production without affecting cAMP accumulation. Moreover, exposure of luteal cells to PLC increased P4 production in a dose-dependent manner. These results support the hypothesis that the luteotropic action of LH in bovine luteal cells is mediated not only by activation of adenylate cyclase but also by activation of PLC.

Original languageEnglish
Pages (from-to)257-261
Number of pages5
JournalJournal of Reproduction and Development
Volume50
Issue number2
DOIs
Publication statusPublished - 2004

Fingerprint

Luteal Cells
luteal cells
phospholipase C
Type C Phospholipases
luteinizing hormone
Luteinizing Hormone
Progesterone
progesterone
cattle
cyclic AMP
steroidogenesis
adenylate cyclase
Estrous Cycle
Cyclic AMP-Dependent Protein Kinases
Adenylyl Cyclases
estrous cycle
protein kinases
Cyclic AMP
cell culture
Cell Culture Techniques

Keywords

  • Corpus luteum
  • Cow
  • Luteinizing hormone
  • Phospholipase-C
  • Progesterone

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Progesterone stimulation by LH involves the phospholipase-C pathway in bovine luteal cells. / Nishimura, Ryo; Shibaya, Masami; Skarzynski, Dariusz J.; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 50, No. 2, 2004, p. 257-261.

Research output: Contribution to journalArticle

Nishimura, Ryo ; Shibaya, Masami ; Skarzynski, Dariusz J. ; Okuda, Kiyoshi. / Progesterone stimulation by LH involves the phospholipase-C pathway in bovine luteal cells. In: Journal of Reproduction and Development. 2004 ; Vol. 50, No. 2. pp. 257-261.
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