TY - JOUR
T1 - Progenitor cells from atria, ventricle and peripheral blood of the same patients exhibit functional differences associated with cardiac repair
AU - Dixit, Parul
AU - Donnelly, Hayden
AU - Edamatsu, Midori
AU - Galvin, Ivor
AU - Bunton, Richard
AU - Katare, Rajesh
N1 - Funding Information:
This study was funded by project grants from J &C Anderson Trust, New Zealand , Heart Foundation New Zealand ( 1560 ) and University of Otago Research Grants . Parul Dixit was supported by a Doctoral scholarship from the University of Otago during the duration of this study.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Aim Deciding the best cell type for cardiac regeneration remains a big challenge. No studies have directly compared the functional efficacy of cardiac progenitor cells (CPCs) with extra-cardiac stem cells isolated from the same patient. Methods and results We compared the functional characteristics of endothelial progenitor cells (EPCs), right atrial (RAA) CPCs and left ventricular (LV) CPCs isolated from the same patients (n = 14). Within the same heart, RAA and LV CPCs exhibited marked differences in surface marker expression, with RAA CPCs exhibiting better expansion potential and migration properties. When subjected to hypoxia and serum starvation to simulate in vivo ischemic environment, RAA and LV CPCs exhibited similar pattern of resistance to apoptotic cell death under ischemia. Interestingly, EPCs exhibited highest resistance to apoptotic cell death, however, they also showed the lowest proliferation under hypoxia. RT-profiler array showed comparable gene expression pattern in RAA and LV CPCs, while they were differentially expressed in EPCs. Further, treating human umbilical vein endothelial cells with conditioned medium (CM) from LV showed maximum angiogenic potential, while cardiomyocytes treated with CM from RAA showed greatest survival under hypoxic conditions. Conclusions Results from this study provide the first evidence that progenitor cells from different regions exhibit functional differences within the same patient.
AB - Aim Deciding the best cell type for cardiac regeneration remains a big challenge. No studies have directly compared the functional efficacy of cardiac progenitor cells (CPCs) with extra-cardiac stem cells isolated from the same patient. Methods and results We compared the functional characteristics of endothelial progenitor cells (EPCs), right atrial (RAA) CPCs and left ventricular (LV) CPCs isolated from the same patients (n = 14). Within the same heart, RAA and LV CPCs exhibited marked differences in surface marker expression, with RAA CPCs exhibiting better expansion potential and migration properties. When subjected to hypoxia and serum starvation to simulate in vivo ischemic environment, RAA and LV CPCs exhibited similar pattern of resistance to apoptotic cell death under ischemia. Interestingly, EPCs exhibited highest resistance to apoptotic cell death, however, they also showed the lowest proliferation under hypoxia. RT-profiler array showed comparable gene expression pattern in RAA and LV CPCs, while they were differentially expressed in EPCs. Further, treating human umbilical vein endothelial cells with conditioned medium (CM) from LV showed maximum angiogenic potential, while cardiomyocytes treated with CM from RAA showed greatest survival under hypoxic conditions. Conclusions Results from this study provide the first evidence that progenitor cells from different regions exhibit functional differences within the same patient.
KW - Angiogenesis
KW - Cardiac regeneration
KW - Source
KW - Stem cells
KW - Survival
KW - Therapy
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U2 - 10.1016/j.ijcard.2016.11.178
DO - 10.1016/j.ijcard.2016.11.178
M3 - Article
C2 - 27875722
AN - SCOPUS:84995772453
VL - 228
SP - 412
EP - 421
JO - International Journal of Cardiology
JF - International Journal of Cardiology
SN - 0167-5273
ER -