Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source

Tatsuo Kawarasaki, Kazuhiko Uchiyama, Atsushi Hirao, Sadahiro Azuma, Masayoshi Otake, Masatoshi Shibata, Seiko Tsuchiya, Shin Enosawa, Koichi Takeuchi, Kenjiro Konno, Yoji Hakamata, Hiroyuki Yoshino, Takuya Wakai, Shigeo Ookawara, Hozumi Tanaka, Eiji Kobayashi, Takashi Murakami

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and highperformance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation.

Original languageEnglish
Article number054017
JournalJournal of Biomedical Optics
Volume14
Issue number5
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

Keywords

  • Biological probes
  • Green fluorescent protein
  • Jinhua pigs
  • Somatic cell cloning

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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  • Cite this

    Kawarasaki, T., Uchiyama, K., Hirao, A., Azuma, S., Otake, M., Shibata, M., Tsuchiya, S., Enosawa, S., Takeuchi, K., Konno, K., Hakamata, Y., Yoshino, H., Wakai, T., Ookawara, S., Tanaka, H., Kobayashi, E., & Murakami, T. (2009). Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source. Journal of Biomedical Optics, 14(5), [054017]. https://doi.org/10.1117/1.3241985