Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries

Takuya Wakai, Satoshi Sugimura, Ken Ichi Yamanaka, Manabu Kawahara, Hiroshi Sasada, Hozumi Tanaka, Asako Ando, Eiji Kobayashi, Eimei Sato

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

For production of viable somatic cell nuclear transferred (SCNT) miniature pig embryos, in vitro condition for controlling the quality of recipient oocytes derived from domestic pig ovaries should be evaluated. In the present study, to get information on optimal in vitro maturation (IVM) condition of oocytes, we investigated the effect of IVM duration of recipient oocytes on subsequent development of SCNT miniature pig embryos, the maturation-promoting factor (MPF) activity in recipient oocytes before and after SCNT, and the occurrence of premature chromosome condensation (PCC) and spindle morphologies of donor nuclei following SCNT. The optimal window of the IVM period in terms of in vitro developmental ability of SCNT embryos was determined to be 36-40 h after the start of IVM. The use of recipient oocytes matured for 36 and 40 h resulted in a high level of MPF activity before and after SCNT, and increased the occurrence of PCC in transferred nuclei compared to the use of oocytes matured for 44 and 52 h. The proportion of abnormal spindle-like structures increased as the IVM period was prolonged. In addition, SCNT embryos constructed from recipient cytoplasts obtained after 40 h of maturation by using fetal fibroblasts of miniature pigs were transferred to surrogate miniature pigs, and developed to full term. These results suggest that recipient oocytes matured for 36 h and 40 h effectively induce PCC with a normal cytoskeletal structure because of a high level of MPF activity; furthermore, the 40-h IVM period improves in vitro development of SCNT embryos to the blastocyst stage, resulting in the production of viable cloned miniature pigs.

Original languageEnglish
Pages (from-to)249-261
Number of pages13
JournalCloning and Stem Cells
Volume10
Issue number2
DOIs
Publication statusPublished - Jun 1 2008
Externally publishedYes

Fingerprint

Sus scrofa
Oocytes
Ovary
Swine
Embryonic Structures
Maturation-Promoting Factor
Chromosomes
In Vitro Oocyte Maturation Techniques
In Vitro Techniques
Blastocyst
Fibroblasts

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Applied Microbiology and Biotechnology

Cite this

Wakai, T., Sugimura, S., Yamanaka, K. I., Kawahara, M., Sasada, H., Tanaka, H., ... Sato, E. (2008). Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries. Cloning and Stem Cells, 10(2), 249-261. https://doi.org/10.1089/clo.2007.0045

Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries. / Wakai, Takuya; Sugimura, Satoshi; Yamanaka, Ken Ichi; Kawahara, Manabu; Sasada, Hiroshi; Tanaka, Hozumi; Ando, Asako; Kobayashi, Eiji; Sato, Eimei.

In: Cloning and Stem Cells, Vol. 10, No. 2, 01.06.2008, p. 249-261.

Research output: Contribution to journalArticle

Wakai, T, Sugimura, S, Yamanaka, KI, Kawahara, M, Sasada, H, Tanaka, H, Ando, A, Kobayashi, E & Sato, E 2008, 'Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries', Cloning and Stem Cells, vol. 10, no. 2, pp. 249-261. https://doi.org/10.1089/clo.2007.0045
Wakai, Takuya ; Sugimura, Satoshi ; Yamanaka, Ken Ichi ; Kawahara, Manabu ; Sasada, Hiroshi ; Tanaka, Hozumi ; Ando, Asako ; Kobayashi, Eiji ; Sato, Eimei. / Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries. In: Cloning and Stem Cells. 2008 ; Vol. 10, No. 2. pp. 249-261.
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