TY - JOUR
T1 - Production of two phosphoproteins from the NS5A region of the hepatitis C viral genome
AU - Kaneko, Takashi
AU - Tanji, Yasunori
AU - Satoh, Shinya
AU - Hijikata, Makolo
AU - Asabe, Shinichi
AU - Kumura, Koichi
AU - Shimotohno, Kunitada
PY - 1994/11/30
Y1 - 1994/11/30
N2 - Hepatitis C virus produces about 12 viral proteins by proteolytic cleavage of the viral polyprotein precursor produced from the largest open reading frame in the viral genome. We have analyzed the production of viral nonstructural proteins with in vivo transient expression system using COS-1 cells. Two proteins, a 56-kDa protein and a 58-kDa protein, were produced from the nonstructural region 5A (NS5A), which has the potential to produce a 49 kDa protein. We showed that these proteins are phosphorylated at the serine residues. The presence of the two proteins was reflected by different degrees of phosphorylation. Moreover, the hyper-phosphorylation of p58 was shown to depend on the presence of NS4A, another hepatitis C virus protein.
AB - Hepatitis C virus produces about 12 viral proteins by proteolytic cleavage of the viral polyprotein precursor produced from the largest open reading frame in the viral genome. We have analyzed the production of viral nonstructural proteins with in vivo transient expression system using COS-1 cells. Two proteins, a 56-kDa protein and a 58-kDa protein, were produced from the nonstructural region 5A (NS5A), which has the potential to produce a 49 kDa protein. We showed that these proteins are phosphorylated at the serine residues. The presence of the two proteins was reflected by different degrees of phosphorylation. Moreover, the hyper-phosphorylation of p58 was shown to depend on the presence of NS4A, another hepatitis C virus protein.
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U2 - 10.1006/bbrc.1994.2667
DO - 10.1006/bbrc.1994.2667
M3 - Article
C2 - 7999043
AN - SCOPUS:0028096675
SN - 0006-291X
VL - 205
SP - 320
EP - 326
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
