@inbook{bdd2c6fe6b474c45b468ec039c4a7fca,
title = "Production of recombinant CCN2 protein in Escherichia coli",
abstract = "Recombinant proteins are important tools for understanding molecular functions in vitro. Recent progress in the generation of recombinant proteins is amazing. However, when we plan to produce them, we should choose the best method according to the nature and the use of the target recombinant protein. Degradation and mis-folding are major problems in producing active recombinant CCN2. The method shown in this chapter describes the appropriate conditions under which we can produce CCN2 and its truncated fragments in Escherichia coli.",
keywords = "Escherichia coli (E. coli), FLAG peptide, FLAG-tag, Histidine 6 -tag, Imidazole, Ni-NTA agarose gel",
author = "Eriko Aoyama and Takako Hattori and Satoshi Kubota and Masaharu Takigawa",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_8",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "77--84",
booktitle = "Methods in Molecular Biology",
}