Production of recombinant CCN2 protein in Escherichia coli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recombinant proteins are important tools for understanding molecular functions in vitro. Recent progress in the generation of recombinant proteins is amazing. However, when we plan to produce them, we should choose the best method according to the nature and the use of the target recombinant protein. Degradation and mis-folding are major problems in producing active recombinant CCN2. The method shown in this chapter describes the appropriate conditions under which we can produce CCN2 and its truncated fragments in Escherichia coli.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages77-84
Number of pages8
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

Fingerprint

Connective Tissue Growth Factor
Recombinant Proteins
Escherichia coli

Keywords

  • Escherichia coli (E. coli)
  • FLAG peptide
  • FLAG-tag
  • Histidine 6 -tag
  • Imidazole
  • Ni-NTA agarose gel

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Aoyama, E., Hattori, T., Kubota, S., & Takigawa, M. (2017). Production of recombinant CCN2 protein in Escherichia coli. In Methods in Molecular Biology (Vol. 1489, pp. 77-84). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_8

Production of recombinant CCN2 protein in Escherichia coli. / Aoyama, Eriko; Hattori, Takako; Kubota, Satoshi; Takigawa, Masaharu.

Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 77-84 (Methods in Molecular Biology; Vol. 1489).

Research output: Chapter in Book/Report/Conference proceedingChapter

Aoyama, E, Hattori, T, Kubota, S & Takigawa, M 2017, Production of recombinant CCN2 protein in Escherichia coli. in Methods in Molecular Biology. vol. 1489, Methods in Molecular Biology, vol. 1489, Humana Press Inc., pp. 77-84. https://doi.org/10.1007/978-1-4939-6430-7_8
Aoyama E, Hattori T, Kubota S, Takigawa M. Production of recombinant CCN2 protein in Escherichia coli. In Methods in Molecular Biology. Vol. 1489. Humana Press Inc. 2017. p. 77-84. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6430-7_8
Aoyama, Eriko ; Hattori, Takako ; Kubota, Satoshi ; Takigawa, Masaharu. / Production of recombinant CCN2 protein in Escherichia coli. Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. pp. 77-84 (Methods in Molecular Biology).
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