Production of novel anti-recombinant human erythropoietin monoclonal antibodies and development of a sensitive enzyme-linked immunosorbent assay for detection of bioactive human erythropoietin

Shigehiro Yanagihara, Yuko Kori, Rika Ishikawa, Kazuhiro Kutsukake

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Erythropoietin (EPO) is a growth factor, regulating the proliferation and differentiation of erythroid progenitor cells. In this study, we generated five monoclonal antibodies (mAbs) that reacted specifically with recombinant human EPO (rhEPO). Epitope exclusion and other experiments showed that the mAbs obtained were divided into two groups, differing in recognition sites for rhEPO: group 1 mAbs recognize the N-terminal region of rhEPO, whereas group 2 mAbs seem to recognize a conformation-dependent epitope. Although most of the previously reported anti-EPO antibodies that recognized the N-terminal region of EPO lacked the EPO-neutralizing activity, the group 1 mAbs obtained here had the rhEPO-neutralizing activity. Therefore, the group 1 mAbs may be useful for future study on structure-function relationship of EPO. One of the group 2 mAbs, 5D11A, showed the highest affinity for rhEPO with KD value 0.52 nM and had the highest rhEPO-neutralizing activity. Using this mAb, we developed a reproducible and sensitive enzyme-linked immunosorbent assay for the quantification of bioactive rhEPO.

Original languageEnglish
Pages (from-to)181-196
Number of pages16
JournalJournal of Immunoassay and Immunochemistry
Volume29
Issue number2
DOIs
Publication statusPublished - Apr 1 2008

Keywords

  • Cell-based potency assay
  • Conformation- dependent epitope
  • Enzyme-linked immunosorbent assay
  • Monoclonal antibodies
  • Recombinant human erythropoietin
  • Validation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Medical Laboratory Technology

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