Production of nitric oxide under Ultraviolet-B irradiation is mediated by hydrogen peroxide through activation of nitric oxide synthase

Lingang Zhang, Liqun Zhao

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    Excised leaves of kidney bean (Phaseolus vulgaris) were used to investigate the mechanism of NO generation under UV-B stress. We showed that two signaling molecules, NO and H2O2, were produced in the irradiated leaves. NO release was blocked by LNNA, an inhibitor of NOS. Application of CAT (EC 1.11.1.6) not only effectively eliminated H2O2 in the leaves, but also inhibited the activity of NOS and the emission of NO. In contrast, treatment with exogenous H2O2 increased both of those events. Therefore, we suggest that, under UV-B stress, NO production is mediated by H2O2 through greater NOS activity.

    Original languageEnglish
    Pages (from-to)395-400
    Number of pages6
    JournalJournal of Plant Biology
    Volume51
    Issue number6
    DOIs
    Publication statusPublished - Nov 2008

    Keywords

    • Catalase
    • Hydrogen peroxide
    • Nitric oxide
    • Nitric oxide synthase
    • Ultraviolet B

    ASJC Scopus subject areas

    • Plant Science

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