Production of nitric oxide under Ultraviolet-B irradiation is mediated by hydrogen peroxide through activation of nitric oxide synthase

Lingang Zhang, Liqun Zhao

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Excised leaves of kidney bean (Phaseolus vulgaris) were used to investigate the mechanism of NO generation under UV-B stress. We showed that two signaling molecules, NO and H2O2, were produced in the irradiated leaves. NO release was blocked by LNNA, an inhibitor of NOS. Application of CAT (EC 1.11.1.6) not only effectively eliminated H2O2 in the leaves, but also inhibited the activity of NOS and the emission of NO. In contrast, treatment with exogenous H2O2 increased both of those events. Therefore, we suggest that, under UV-B stress, NO production is mediated by H2O2 through greater NOS activity.

Original languageEnglish
Pages (from-to)395-400
Number of pages6
JournalJournal of Plant Biology
Volume51
Issue number6
DOIs
Publication statusPublished - Nov 2008
Externally publishedYes

Fingerprint

nitric oxide synthase
nitric oxide
ultraviolet radiation
hydrogen peroxide
irradiation
leaves
kidney beans
Phaseolus vulgaris
catalase

Keywords

  • Catalase
  • Hydrogen peroxide
  • Nitric oxide
  • Nitric oxide synthase
  • Ultraviolet B

ASJC Scopus subject areas

  • Plant Science

Cite this

Production of nitric oxide under Ultraviolet-B irradiation is mediated by hydrogen peroxide through activation of nitric oxide synthase. / Zhang, Lingang; Zhao, Liqun.

In: Journal of Plant Biology, Vol. 51, No. 6, 11.2008, p. 395-400.

Research output: Contribution to journalArticle

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