Production of murine leukemia RL♂1 rejection antigen peptide pRL1a by proteolysis of natural precursor pRL1b

Toshiro Ono, Akiko Uenaka, Eiichi Nakayama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, we demonstrated that NH2-terminal Ser and Ile residues of pRL1b (SI-pRL1a) (SIIPGLPLSL) are not involved in the recognition by RL♂l-specific cytotoxic T lymphocyte. The sensitization activity observed with pRL1b (SI-pRL1a) was not greater than that of peptides substituted with irrelevant amino acids at these positions. In serum-free medium, pRL1a retained sensitization activity, but pRL1b (SI-pRL1a) did not. Furthermore, addition of bestatin to serum-containing medium blocked sensitization by pRL1b (SI-pRL1a). On the other hand, the addition of captopril enhanced it, probably by inhibiting the degradation of pRL1a by ACE, pRL1a-D peptide with D-Ile in place of the L-Ile residue of pRL1a (IPGLPLSL) showed sensitization, but SI-pRL1a2,3D peptide, which has D-Iles in place of the L-Ile residues of pRL1b (SI-pRL1a), and which was not cleaved between the two D-Iles, did not. The findings suggest that pRL1a is the antigenic peptide bound to L(d) molecules and pRL1b (SI-pRL1a) peptide is its natural precursor, which generates pRL1a via proteolysis.

Original languageEnglish
Pages (from-to)1165-1170
Number of pages6
JournalJapanese Journal of Cancer Research
Volume87
Issue number11
Publication statusPublished - Nov 1996

Fingerprint

Proteolysis
Leukemia
Antigens
Peptides
Captopril
Serum-Free Culture Media
Cytotoxic T-Lymphocytes
Amino Acids
Serum

Keywords

  • Balb/c leukemia RL♂1
  • Cytotoxic T lymphocyte
  • Natural precursor peptide
  • Protease
  • Tumor rejection antigen peptide

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Production of murine leukemia RL♂1 rejection antigen peptide pRL1a by proteolysis of natural precursor pRL1b. / Ono, Toshiro; Uenaka, Akiko; Nakayama, Eiichi.

In: Japanese Journal of Cancer Research, Vol. 87, No. 11, 11.1996, p. 1165-1170.

Research output: Contribution to journalArticle

Ono, Toshiro ; Uenaka, Akiko ; Nakayama, Eiichi. / Production of murine leukemia RL♂1 rejection antigen peptide pRL1a by proteolysis of natural precursor pRL1b. In: Japanese Journal of Cancer Research. 1996 ; Vol. 87, No. 11. pp. 1165-1170.
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