Production of monocyte chemotactic protein-1 by rat brain macrophages

C. F. Calvo, Teizo Yoshimura, M. Gelman, M. Mallat

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

In the present study, we show that cultured rat brain macrophages release a soluble factor that stimulates the migration of bone marrow-derived macrophages, as determined by an in vitro chemotaxis assay. A checkerboard analysis indicated that most of this effect resulted from a polarized migration of the cells (chemotactic phenomenon), rather than in an increase in cell motility (chemokinesis). This activity was significantly decreased by an immune serum directed against the rat monocyte chemoattractant protein-1 (chemokine MCP-1). Northern blot analysis demonstrated expression of the MCP-1 gene in cultured brain macrophages, but its absence in unstimulated bone marrow-derived macrophages. Up-regulation of MCP-1 expression was observed when lipopolysaccharide was added to cultured brain macrophages, a peak occurring after a 6 h period of stimulation. Also, inflammatory cytokines such as interleukin (IL)-1β, colony stimulating factor-1, tumour necrosis factor-or and IL-6 individually increased the basal level of MCP-1 mRNA. Subsequently, we demonstrated the in vivo production of MCP-1 in the adult rat brain following injury induced by a local injection of kainic acid. MCP-1 synthesis was localized in both astrocytes and brain macrophages. These results suggest that the activation of resting microglial cells into brain macrophages and their subsequent secretion of chemokines could contribute to the mechanism(s), leading to the infiltration of the CNS by blood-derived monocytes, as observed in several pathologies.

Original languageEnglish
Pages (from-to)1725-1734
Number of pages10
JournalEuropean Journal of Neuroscience
Volume8
Issue number8
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Macrophages
Brain
Chemokines
Cell Movement
Macrophage Colony-Stimulating Factor
Kainic Acid
Chemotaxis
rat Ccl2 protein
Interleukin-1
Astrocytes
Northern Blotting
Brain Injuries
Lipopolysaccharides
Immune Sera
Monocytes
Interleukin-6
Up-Regulation
Tumor Necrosis Factor-alpha
Pathology
Cytokines

Keywords

  • Brain injury
  • Chemotactic migration
  • Inflammatory cytokines
  • Microglia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Production of monocyte chemotactic protein-1 by rat brain macrophages. / Calvo, C. F.; Yoshimura, Teizo; Gelman, M.; Mallat, M.

In: European Journal of Neuroscience, Vol. 8, No. 8, 1996, p. 1725-1734.

Research output: Contribution to journalArticle

Calvo, C. F. ; Yoshimura, Teizo ; Gelman, M. ; Mallat, M. / Production of monocyte chemotactic protein-1 by rat brain macrophages. In: European Journal of Neuroscience. 1996 ; Vol. 8, No. 8. pp. 1725-1734.
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