Production of monocyte chemoattractant protein-1 by malignant fibrous histiocytoma: Relation to the origin of histiocyte-like cells

Motohiro Takeya, Teizo Yoshimura, Edward J. Leonard, Teiji Kato, Hidetoshi Okabe, Kiyoshi Takahashi

Research output: Contribution to journalArticle

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Abstract

Human malignant fibrous histiocytoma (MFH) comprise both fibroblast-like cells and histiocyte-like cells. We previously showed that the latter are not neoplastic cells, but are infiltrating macrophages. Since migration of blood monocytes into the tumor might be a response to a locally elaborated monocyte chemoattractant, we designed experiments to determine if the fibroblast-like tumor cells produced a chemoattractant for human monocytes. Malignant fibrous histiocytoma from three patients was put into culture. Cells of all three lines had a spindle shape, and showed no reactivity with antibodies against macrophages (MAC387), HLA-DR (LN3), or leukocyte common antigen. Immunohistochemically, they stained with antibody against human monocyte chemoattractant protein-1 (MCP-1). Culture supernatants of the three cell lines had chemotactic activity for monocytes. This activity was due to MCP-1, since it was absorbed by an anti-MCP-1 column. The production of MCP-1 by MFH tumor lines was confirmed by immunoprecipitation of metabolically labeled MCP-1. These results suggest that the histiocyte-like cells are the infiltrated macrophages that originate from blood monocytes attracted by tumor-derived MCP-1.

Original languageEnglish
Pages (from-to)61-71
Number of pages11
JournalExperimental and Molecular Pathology
Volume54
Issue number1
DOIs
Publication statusPublished - 1991
Externally publishedYes

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Malignant Fibrous Histiocytoma
Histiocytes
Chemokine CCL2
Monocytes
Tumors
Macrophages
Chemotactic Factors
Fibroblasts
Blood
Cells
Neoplasms
CD45 Antigens
Antibodies
HLA-DR Antigens
Cell culture
Immunoprecipitation
Cell Line
Experiments

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Production of monocyte chemoattractant protein-1 by malignant fibrous histiocytoma : Relation to the origin of histiocyte-like cells. / Takeya, Motohiro; Yoshimura, Teizo; Leonard, Edward J.; Kato, Teiji; Okabe, Hidetoshi; Takahashi, Kiyoshi.

In: Experimental and Molecular Pathology, Vol. 54, No. 1, 1991, p. 61-71.

Research output: Contribution to journalArticle

Takeya, Motohiro ; Yoshimura, Teizo ; Leonard, Edward J. ; Kato, Teiji ; Okabe, Hidetoshi ; Takahashi, Kiyoshi. / Production of monocyte chemoattractant protein-1 by malignant fibrous histiocytoma : Relation to the origin of histiocyte-like cells. In: Experimental and Molecular Pathology. 1991 ; Vol. 54, No. 1. pp. 61-71.
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