Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells

Y. Kakizaki, S. Waga, K. Sugimoto, H. Tanaka, K. Nukii, M. Takeya, Teizo Yoshimura, M. Yokoyama

Research output: Contribution to journalArticle

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Abstract

To explore the role of glomerular endothelial cells (GEN) in the pathogenesis of glomerulonephritis, the in vitro production of monocyte chemoattractant protein-1 (MCP-1) by bovine GEN was determined by chemotaxis assay, Northern and Western blot analysis, and immunocytochemistry. Monocyte chemotactic activity of GEN-conditioned media was detectable by a chemotaxis assay using human peripheral blood monocytes. Exposure to human recombinant interleukin-1β (IL-1β) and phorbol myristate acetate (PMA) significantly increased the chemotactic activity of GEN-conditioned media. A checkerboard analysis showed that the response of monocytes to GEN-conditioned media was truly chemotactic. Immunoadsorption with a monoclonal antibody to human MCP-1 reduced the chemotactic activity of GEN-conditioned media by 85%. Northern blot analysis revealed that MCP-1 mRNA was constitutively expressed by GEN and that IL-1β and tumor necrosis factor-α (TNF-α) increased MCP-1 mRNA levels in a dose- and time-dependent manner. Furthermore, PMA induced an increase in MCP-1 mRNA levels, whereas dibutyryl cyclic AMP and forskolin had minimal effects. Inhibition study using protein kinase inhibitors revealed that MCP-1 mRNA expression induced by IL-1β and TNF-α was suppressed by the tyrosine kinase inhibitor genistein, not by the protein kinase C inhibitors staurosporine or H-7, or the protein kinase A inhibitor H-89, suggesting an important role of tyrosine kinase in the cytokine-induced MCP-1 gene expression. Dexamethasone had a small inhibitory effect on constitutive MCP-1 mRNA expression, but no effect on the induction by TNF-α. By immunoperoxidase staining and Western blot analysis using an anti-MCP-1 monoclonal antibody, MCP-1 protein was detected in untreated GEN and increased by exposure to TNF-α. These results demonstrate the cytokine-induced production of MCP-1 by GEN at gene and protein levels as well as bioactivity, and suggest that GEN may participate in the development of glomerulonephritis through the production of MCP-1.

Original languageEnglish
Pages (from-to)1866-1874
Number of pages9
JournalKidney International
Volume48
Issue number6
Publication statusPublished - 1995
Externally publishedYes

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Chemokine CCL2
Endothelial Cells
Conditioned Culture Medium
Protein Kinase Inhibitors
Messenger RNA
Tumor Necrosis Factor-alpha
Interleukin-1
Monocytes
Tetradecanoylphorbol Acetate
Chemotaxis
Glomerulonephritis
Northern Blotting
Protein-Tyrosine Kinases
Western Blotting
Monoclonal Antibodies
Cytokines
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Bucladesine
Staurosporine
Protein C Inhibitor

ASJC Scopus subject areas

  • Nephrology

Cite this

Kakizaki, Y., Waga, S., Sugimoto, K., Tanaka, H., Nukii, K., Takeya, M., ... Yokoyama, M. (1995). Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells. Kidney International, 48(6), 1866-1874.

Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells. / Kakizaki, Y.; Waga, S.; Sugimoto, K.; Tanaka, H.; Nukii, K.; Takeya, M.; Yoshimura, Teizo; Yokoyama, M.

In: Kidney International, Vol. 48, No. 6, 1995, p. 1866-1874.

Research output: Contribution to journalArticle

Kakizaki, Y, Waga, S, Sugimoto, K, Tanaka, H, Nukii, K, Takeya, M, Yoshimura, T & Yokoyama, M 1995, 'Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells', Kidney International, vol. 48, no. 6, pp. 1866-1874.
Kakizaki Y, Waga S, Sugimoto K, Tanaka H, Nukii K, Takeya M et al. Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells. Kidney International. 1995;48(6):1866-1874.
Kakizaki, Y. ; Waga, S. ; Sugimoto, K. ; Tanaka, H. ; Nukii, K. ; Takeya, M. ; Yoshimura, Teizo ; Yokoyama, M. / Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells. In: Kidney International. 1995 ; Vol. 48, No. 6. pp. 1866-1874.
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abstract = "To explore the role of glomerular endothelial cells (GEN) in the pathogenesis of glomerulonephritis, the in vitro production of monocyte chemoattractant protein-1 (MCP-1) by bovine GEN was determined by chemotaxis assay, Northern and Western blot analysis, and immunocytochemistry. Monocyte chemotactic activity of GEN-conditioned media was detectable by a chemotaxis assay using human peripheral blood monocytes. Exposure to human recombinant interleukin-1β (IL-1β) and phorbol myristate acetate (PMA) significantly increased the chemotactic activity of GEN-conditioned media. A checkerboard analysis showed that the response of monocytes to GEN-conditioned media was truly chemotactic. Immunoadsorption with a monoclonal antibody to human MCP-1 reduced the chemotactic activity of GEN-conditioned media by 85{\%}. Northern blot analysis revealed that MCP-1 mRNA was constitutively expressed by GEN and that IL-1β and tumor necrosis factor-α (TNF-α) increased MCP-1 mRNA levels in a dose- and time-dependent manner. Furthermore, PMA induced an increase in MCP-1 mRNA levels, whereas dibutyryl cyclic AMP and forskolin had minimal effects. Inhibition study using protein kinase inhibitors revealed that MCP-1 mRNA expression induced by IL-1β and TNF-α was suppressed by the tyrosine kinase inhibitor genistein, not by the protein kinase C inhibitors staurosporine or H-7, or the protein kinase A inhibitor H-89, suggesting an important role of tyrosine kinase in the cytokine-induced MCP-1 gene expression. Dexamethasone had a small inhibitory effect on constitutive MCP-1 mRNA expression, but no effect on the induction by TNF-α. By immunoperoxidase staining and Western blot analysis using an anti-MCP-1 monoclonal antibody, MCP-1 protein was detected in untreated GEN and increased by exposure to TNF-α. These results demonstrate the cytokine-induced production of MCP-1 by GEN at gene and protein levels as well as bioactivity, and suggest that GEN may participate in the development of glomerulonephritis through the production of MCP-1.",
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N2 - To explore the role of glomerular endothelial cells (GEN) in the pathogenesis of glomerulonephritis, the in vitro production of monocyte chemoattractant protein-1 (MCP-1) by bovine GEN was determined by chemotaxis assay, Northern and Western blot analysis, and immunocytochemistry. Monocyte chemotactic activity of GEN-conditioned media was detectable by a chemotaxis assay using human peripheral blood monocytes. Exposure to human recombinant interleukin-1β (IL-1β) and phorbol myristate acetate (PMA) significantly increased the chemotactic activity of GEN-conditioned media. A checkerboard analysis showed that the response of monocytes to GEN-conditioned media was truly chemotactic. Immunoadsorption with a monoclonal antibody to human MCP-1 reduced the chemotactic activity of GEN-conditioned media by 85%. Northern blot analysis revealed that MCP-1 mRNA was constitutively expressed by GEN and that IL-1β and tumor necrosis factor-α (TNF-α) increased MCP-1 mRNA levels in a dose- and time-dependent manner. Furthermore, PMA induced an increase in MCP-1 mRNA levels, whereas dibutyryl cyclic AMP and forskolin had minimal effects. Inhibition study using protein kinase inhibitors revealed that MCP-1 mRNA expression induced by IL-1β and TNF-α was suppressed by the tyrosine kinase inhibitor genistein, not by the protein kinase C inhibitors staurosporine or H-7, or the protein kinase A inhibitor H-89, suggesting an important role of tyrosine kinase in the cytokine-induced MCP-1 gene expression. Dexamethasone had a small inhibitory effect on constitutive MCP-1 mRNA expression, but no effect on the induction by TNF-α. By immunoperoxidase staining and Western blot analysis using an anti-MCP-1 monoclonal antibody, MCP-1 protein was detected in untreated GEN and increased by exposure to TNF-α. These results demonstrate the cytokine-induced production of MCP-1 by GEN at gene and protein levels as well as bioactivity, and suggest that GEN may participate in the development of glomerulonephritis through the production of MCP-1.

AB - To explore the role of glomerular endothelial cells (GEN) in the pathogenesis of glomerulonephritis, the in vitro production of monocyte chemoattractant protein-1 (MCP-1) by bovine GEN was determined by chemotaxis assay, Northern and Western blot analysis, and immunocytochemistry. Monocyte chemotactic activity of GEN-conditioned media was detectable by a chemotaxis assay using human peripheral blood monocytes. Exposure to human recombinant interleukin-1β (IL-1β) and phorbol myristate acetate (PMA) significantly increased the chemotactic activity of GEN-conditioned media. A checkerboard analysis showed that the response of monocytes to GEN-conditioned media was truly chemotactic. Immunoadsorption with a monoclonal antibody to human MCP-1 reduced the chemotactic activity of GEN-conditioned media by 85%. Northern blot analysis revealed that MCP-1 mRNA was constitutively expressed by GEN and that IL-1β and tumor necrosis factor-α (TNF-α) increased MCP-1 mRNA levels in a dose- and time-dependent manner. Furthermore, PMA induced an increase in MCP-1 mRNA levels, whereas dibutyryl cyclic AMP and forskolin had minimal effects. Inhibition study using protein kinase inhibitors revealed that MCP-1 mRNA expression induced by IL-1β and TNF-α was suppressed by the tyrosine kinase inhibitor genistein, not by the protein kinase C inhibitors staurosporine or H-7, or the protein kinase A inhibitor H-89, suggesting an important role of tyrosine kinase in the cytokine-induced MCP-1 gene expression. Dexamethasone had a small inhibitory effect on constitutive MCP-1 mRNA expression, but no effect on the induction by TNF-α. By immunoperoxidase staining and Western blot analysis using an anti-MCP-1 monoclonal antibody, MCP-1 protein was detected in untreated GEN and increased by exposure to TNF-α. These results demonstrate the cytokine-induced production of MCP-1 by GEN at gene and protein levels as well as bioactivity, and suggest that GEN may participate in the development of glomerulonephritis through the production of MCP-1.

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