Production of monoclonal antibodies to human T-cell lymphotropic virus type 1 (HTLV-1) and studies of their specificity

K. Iwatsuki, F. X. Jin, M. Takigawa, K. Sugaya, M. Yamada

Research output: Contribution to journalArticle

Abstract

Mouse monoclonal antibodies to HTLV-1 core proteins, p19 and p24, were obtained by hybridoma technique. The specificity was studied by indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA), dot blot, and Western blot studies. Polyclonal antibodies reactive with HTLV-1 viral proteins were raised by rabbits and guinea pigs. A sandwich ELISA and a dot blot method using these antibodies detected the soluble viral antigens in the ATL cell lysate and ATL culture media. No reaction was observed when IL-2-activated human T-cell lysate and the culture media were used as controls. These data showed that our sandwich ELISA and dot blot systems are available for quantitative analysis of the soluble HTLV-1 viral antigens and contribute to understanding the ability of viral replication by ATL cells.

Original languageEnglish
Pages (from-to)1059-1065
Number of pages7
JournalNippon Hifuka Gakkai zasshi. The Japanese journal of dermatology
Volume99
Issue number10
Publication statusPublished - Sep 1989
Externally publishedYes

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Human T-lymphotropic virus 1
Monoclonal Antibodies
T-Lymphocytes
Viral Antigens
Enzyme-Linked Immunosorbent Assay
Culture Media
Viral Core Proteins
Antibodies
Hybridomas
Viral Proteins
Indirect Fluorescent Antibody Technique
Interleukin-2
Guinea Pigs
Cell Culture Techniques
Western Blotting
Rabbits

ASJC Scopus subject areas

  • Medicine(all)

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Production of monoclonal antibodies to human T-cell lymphotropic virus type 1 (HTLV-1) and studies of their specificity. / Iwatsuki, K.; Jin, F. X.; Takigawa, M.; Sugaya, K.; Yamada, M.

In: Nippon Hifuka Gakkai zasshi. The Japanese journal of dermatology, Vol. 99, No. 10, 09.1989, p. 1059-1065.

Research output: Contribution to journalArticle

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