Abstract
The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 322-326 |
| Number of pages | 5 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology (medical)
- Microbiology
- Bioengineering
- Biotechnology
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Production of l-cystathionine using bacterial cystathionine γ-sythase. / Kanzaki, Hiroshi; Kobayashi, Michihiko; Nagasawa, Toru; Yamada, Hideaki.
In: Applied Microbiology and Biotechnology, Vol. 25, No. 4, 01.1987, p. 322-326.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of l-cystathionine using bacterial cystathionine γ-sythase
AU - Kanzaki, Hiroshi
AU - Kobayashi, Michihiko
AU - Nagasawa, Toru
AU - Yamada, Hideaki
PY - 1987/1
Y1 - 1987/1
N2 - The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.
AB - The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0023134465&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023134465&partnerID=8YFLogxK
U2 - 10.1007/BF00252541
DO - 10.1007/BF00252541
M3 - Article
VL - 25
SP - 322
EP - 326
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 4
ER -