Production of l-cystathionine using bacterial cystathionine γ-sythase

Hiroshi Kanzaki; Michihiko Kobayashi; Toru Nagasawa; Hideaki Yamada

doi:10.1007/BF00252541

Production of l-cystathionine using bacterial cystathionine γ-sythase

Hiroshi Kanzaki, Michihiko Kobayashi, Toru Nagasawa, Hideaki Yamada

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.

Original language	English
Pages (from-to)	322-326
Number of pages	5
Journal	Applied Microbiology and Biotechnology
Volume	25
Issue number	4
DOIs	https://doi.org/10.1007/BF00252541
Publication status	Published - Jan 1 1987
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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abstract = "The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.",

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T1 - Production of l-cystathionine using bacterial cystathionine γ-sythase

AU - Kanzaki, Hiroshi

AU - Kobayashi, Michihiko

AU - Nagasawa, Toru

AU - Yamada, Hideaki

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.

AB - The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.

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