Production of l-cystathionine using bacterial cystathionine γ-sythase

Hiroshi Kanzaki; Michihiko Kobayashi; Toru Nagasawa; Hideaki Yamada

doi:10.1007/BF00252541

Production of l-cystathionine using bacterial cystathionine γ-sythase

Hiroshi Kanzaki, Michihiko Kobayashi, Toru Nagasawa, Hideaki Yamada

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.

Original language	English
Pages (from-to)	322-326
Number of pages	5
Journal	Applied Microbiology and Biotechnology
Volume	25
Issue number	4
DOIs	https://doi.org/10.1007/BF00252541
Publication status	Published - Jan 1987
Externally published	Yes

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ASJC Scopus subject areas

Microbiology (medical)
Microbiology
Bioengineering
Biotechnology

Cite this

Kanzaki, H., Kobayashi, M., Nagasawa, T., & Yamada, H. (1987). Production of l-cystathionine using bacterial cystathionine γ-sythase. Applied Microbiology and Biotechnology, 25(4), 322-326. https://doi.org/10.1007/BF00252541

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AB - The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.

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10.1007/BF00252541