Production of l-cystathionine using bacterial cystathionine γ-sythase

Hiroshi Kanzaki, Michihiko Kobayashi, Toru Nagasawa, Hideaki Yamada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.

Original languageEnglish
Pages (from-to)322-326
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume25
Issue number4
DOIs
Publication statusPublished - Jan 1987
Externally publishedYes

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Cystathionine
Bacilli
Bacillus
Pectobacterium carotovorum
Erwinia
Glycogen Synthase
Enzymes
Cell Extracts

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Bioengineering
  • Biotechnology

Cite this

Production of l-cystathionine using bacterial cystathionine γ-sythase. / Kanzaki, Hiroshi; Kobayashi, Michihiko; Nagasawa, Toru; Yamada, Hideaki.

In: Applied Microbiology and Biotechnology, Vol. 25, No. 4, 01.1987, p. 322-326.

Research output: Contribution to journalArticle

Kanzaki, Hiroshi ; Kobayashi, Michihiko ; Nagasawa, Toru ; Yamada, Hideaki. / Production of l-cystathionine using bacterial cystathionine γ-sythase. In: Applied Microbiology and Biotechnology. 1987 ; Vol. 25, No. 4. pp. 322-326.
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