Production of ergothioneine by Methylobacterium species

Kabir M. Alamgir, Sachiko Masuda, Yoshiko Fujitani, Fumio Fukuda, Akio Tani

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is a sulfur-containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy and physiology for plant surface-symbiotic Methylobacterium species were investigated in this study. Almost all Methylobacterium type strains can synthesize EGT. We selected one of the most productive strains (M. aquaticum strain 22A isolated from a moss), and investigated the feasibility of fermentative EGT production through optimization of the culture condition. Methanol as a carbon source served as the best substrate for production. The productivity reached up to 1000 μg/100 ml culture (1200 μg/g wet weight cells, 6.3 mg/g dry weight) in 38 days. Next, we identified the genes (egtBD) responsible for EGT synthesis, and generated a deletion mutant defective in EGT production. Compared to the wild type, the mutant showed better growth on methanol and on the plant surface as well as severe susceptibility to heat treatment and irradiation of ultraviolet (UV) and sunlight. These results suggested that EGT is not involved in methylotrophy, but is involved in their phyllospheric lifestyle fitness of the genus in natural conditions.

Original languageEnglish
Article number01185
JournalFrontiers in Microbiology
Volume6
Issue numberOCT
DOIs
Publication statusPublished - 2015

Fingerprint

Ergothioneine
Methylobacterium
Methanol
Plant Physiological Phenomena
Bryophyta
Weights and Measures
Metabolomics
Sunlight
Sulfur
Histidine
Life Style
Carbon
Hot Temperature
Amino Acids

Keywords

  • Antioxidant
  • Ergothioneine
  • Glutathione
  • Methanol
  • Methylobacterium species
  • Methylotroph
  • Reactive oxygen species

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Production of ergothioneine by Methylobacterium species. / Alamgir, Kabir M.; Masuda, Sachiko; Fujitani, Yoshiko; Fukuda, Fumio; Tani, Akio.

In: Frontiers in Microbiology, Vol. 6, No. OCT, 01185, 2015.

Research output: Contribution to journalArticle

Alamgir, Kabir M. ; Masuda, Sachiko ; Fujitani, Yoshiko ; Fukuda, Fumio ; Tani, Akio. / Production of ergothioneine by Methylobacterium species. In: Frontiers in Microbiology. 2015 ; Vol. 6, No. OCT.
@article{a892196d9f50476f96e828378ce35643,
title = "Production of ergothioneine by Methylobacterium species",
abstract = "Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is a sulfur-containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy and physiology for plant surface-symbiotic Methylobacterium species were investigated in this study. Almost all Methylobacterium type strains can synthesize EGT. We selected one of the most productive strains (M. aquaticum strain 22A isolated from a moss), and investigated the feasibility of fermentative EGT production through optimization of the culture condition. Methanol as a carbon source served as the best substrate for production. The productivity reached up to 1000 μg/100 ml culture (1200 μg/g wet weight cells, 6.3 mg/g dry weight) in 38 days. Next, we identified the genes (egtBD) responsible for EGT synthesis, and generated a deletion mutant defective in EGT production. Compared to the wild type, the mutant showed better growth on methanol and on the plant surface as well as severe susceptibility to heat treatment and irradiation of ultraviolet (UV) and sunlight. These results suggested that EGT is not involved in methylotrophy, but is involved in their phyllospheric lifestyle fitness of the genus in natural conditions.",
keywords = "Antioxidant, Ergothioneine, Glutathione, Methanol, Methylobacterium species, Methylotroph, Reactive oxygen species",
author = "Alamgir, {Kabir M.} and Sachiko Masuda and Yoshiko Fujitani and Fumio Fukuda and Akio Tani",
year = "2015",
doi = "10.3389/fmicb.2015.01185",
language = "English",
volume = "6",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
number = "OCT",

}

TY - JOUR

T1 - Production of ergothioneine by Methylobacterium species

AU - Alamgir, Kabir M.

AU - Masuda, Sachiko

AU - Fujitani, Yoshiko

AU - Fukuda, Fumio

AU - Tani, Akio

PY - 2015

Y1 - 2015

N2 - Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is a sulfur-containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy and physiology for plant surface-symbiotic Methylobacterium species were investigated in this study. Almost all Methylobacterium type strains can synthesize EGT. We selected one of the most productive strains (M. aquaticum strain 22A isolated from a moss), and investigated the feasibility of fermentative EGT production through optimization of the culture condition. Methanol as a carbon source served as the best substrate for production. The productivity reached up to 1000 μg/100 ml culture (1200 μg/g wet weight cells, 6.3 mg/g dry weight) in 38 days. Next, we identified the genes (egtBD) responsible for EGT synthesis, and generated a deletion mutant defective in EGT production. Compared to the wild type, the mutant showed better growth on methanol and on the plant surface as well as severe susceptibility to heat treatment and irradiation of ultraviolet (UV) and sunlight. These results suggested that EGT is not involved in methylotrophy, but is involved in their phyllospheric lifestyle fitness of the genus in natural conditions.

AB - Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is a sulfur-containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy and physiology for plant surface-symbiotic Methylobacterium species were investigated in this study. Almost all Methylobacterium type strains can synthesize EGT. We selected one of the most productive strains (M. aquaticum strain 22A isolated from a moss), and investigated the feasibility of fermentative EGT production through optimization of the culture condition. Methanol as a carbon source served as the best substrate for production. The productivity reached up to 1000 μg/100 ml culture (1200 μg/g wet weight cells, 6.3 mg/g dry weight) in 38 days. Next, we identified the genes (egtBD) responsible for EGT synthesis, and generated a deletion mutant defective in EGT production. Compared to the wild type, the mutant showed better growth on methanol and on the plant surface as well as severe susceptibility to heat treatment and irradiation of ultraviolet (UV) and sunlight. These results suggested that EGT is not involved in methylotrophy, but is involved in their phyllospheric lifestyle fitness of the genus in natural conditions.

KW - Antioxidant

KW - Ergothioneine

KW - Glutathione

KW - Methanol

KW - Methylobacterium species

KW - Methylotroph

KW - Reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=84946842840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946842840&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2015.01185

DO - 10.3389/fmicb.2015.01185

M3 - Article

VL - 6

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - OCT

M1 - 01185

ER -