TY - JOUR
T1 - Production of colony-stimulating factor in human dental pulp fibroblasts
AU - Sawa, Y.
AU - Horie, Y.
AU - Yamaoka, Y.
AU - Ebata, N.
AU - Kim, T.
AU - Yoshida, S.
PY - 2003/2
Y1 - 2003/2
N2 - Class II major histocompatilibity complex (MHC)-expressing cells are usually distributed in dental pulp, and it was postulated that the colony-stimulating factor (CSF) derived from dental pulp fibroblasts contributes to the migration of class II MHC-expressing cells into pulp tissue. This study aimed to investigate the CSF production of human denial pulp fibroblasts. In pulp tissue sections, granulocyte (G)-CSF was detected from normal teeth, while G-CSF, macrophage (M)-CSF, and granulocyte-macrophage (GM)-CSF were detected from teeth with dentinal caries. In cultured dental pulp fibroblasts, G-CSF was detected by immunostaining, immunoprecipitation, and ELISA, and mRNAs of G-CSF, M-CSF, and GM-CSF were detected by RT-PCR. The dental pulp fibroblasts cultured with TNF-α were found to increase the G-CSF expression and to produce M-CSF and GM-CSF. These findings suggest that dental pulp fibroblasts usually produce G-CSF. In the presence of TNF-α, dental pulp fibroblast express M-CSF and GM-CSF.
AB - Class II major histocompatilibity complex (MHC)-expressing cells are usually distributed in dental pulp, and it was postulated that the colony-stimulating factor (CSF) derived from dental pulp fibroblasts contributes to the migration of class II MHC-expressing cells into pulp tissue. This study aimed to investigate the CSF production of human denial pulp fibroblasts. In pulp tissue sections, granulocyte (G)-CSF was detected from normal teeth, while G-CSF, macrophage (M)-CSF, and granulocyte-macrophage (GM)-CSF were detected from teeth with dentinal caries. In cultured dental pulp fibroblasts, G-CSF was detected by immunostaining, immunoprecipitation, and ELISA, and mRNAs of G-CSF, M-CSF, and GM-CSF were detected by RT-PCR. The dental pulp fibroblasts cultured with TNF-α were found to increase the G-CSF expression and to produce M-CSF and GM-CSF. These findings suggest that dental pulp fibroblasts usually produce G-CSF. In the presence of TNF-α, dental pulp fibroblast express M-CSF and GM-CSF.
KW - Dental pulp
KW - Fibroblasts
KW - G-CSF
KW - GM-CSF
KW - M-CSF
UR - http://www.scopus.com/inward/record.url?scp=0037313024&partnerID=8YFLogxK
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U2 - 10.1177/154405910308200204
DO - 10.1177/154405910308200204
M3 - Article
C2 - 12562880
AN - SCOPUS:0037313024
VL - 82
SP - 96
EP - 100
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - 2
ER -