Production of colony-stimulating factor in human dental pulp fibroblasts

Y. Sawa, Y. Horie, Y. Yamaoka, N. Ebata, T. Kim, S. Yoshida

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15 Citations (Scopus)


Class II major histocompatilibity complex (MHC)-expressing cells are usually distributed in dental pulp, and it was postulated that the colony-stimulating factor (CSF) derived from dental pulp fibroblasts contributes to the migration of class II MHC-expressing cells into pulp tissue. This study aimed to investigate the CSF production of human denial pulp fibroblasts. In pulp tissue sections, granulocyte (G)-CSF was detected from normal teeth, while G-CSF, macrophage (M)-CSF, and granulocyte-macrophage (GM)-CSF were detected from teeth with dentinal caries. In cultured dental pulp fibroblasts, G-CSF was detected by immunostaining, immunoprecipitation, and ELISA, and mRNAs of G-CSF, M-CSF, and GM-CSF were detected by RT-PCR. The dental pulp fibroblasts cultured with TNF-α were found to increase the G-CSF expression and to produce M-CSF and GM-CSF. These findings suggest that dental pulp fibroblasts usually produce G-CSF. In the presence of TNF-α, dental pulp fibroblast express M-CSF and GM-CSF.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalJournal of dental research
Issue number2
Publication statusPublished - Feb 2003
Externally publishedYes


  • Dental pulp
  • Fibroblasts
  • G-CSF
  • GM-CSF
  • M-CSF

ASJC Scopus subject areas

  • Dentistry(all)


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