Production of calves by the transfer of cryopreserved bovine elongating conceptuses and possible application for preimplantation genomic selection

Takashi Fujii, Hiroki Hirayama, Akira Naito, Masashi Kashima, Hitomi Sakai, Shigeo Fukuda, Hitomi Yoshino, Satoru Moriyasu, Soichi Kageyama, Yoshikazu Sugimoto, Shuichi Matsuyama, Hiroyuki Hayakawa, Koji Kimura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Preimplantation genomic selection based on single nucleotide polymorphism (SNP) genotypes is expected to accelerate genetic improvement in cattle. However, genome-wide genotyping at the early embryonic stage has several limitations, such as the technical difficulty of embryonic biopsy and low accuracy of genotyping resulting from a limited number of biopsied cells. After hatching from the zona pellucida, the morphology of the bovine embryo changes from spherical to filamentous, in a process known as elongation. The bovine nonsurgical elongating conceptus transfer technique was recently developed and applied for sexing without requiring specialized skills for biopsy. In order to develop a bovine preimplantation genomic selection system combined with the elongating conceptus transfer technique, we examined the accuracy of genotyping by SNP chip analysis using the DNA from elongating conceptuses (Experiment 1) and optimal cryopreservation methods for elongating conceptuses (Experiment 2). In Experiment 1, the call rates of SNP chip analysis following whole genome amplification in biopsied cells from two elongating conceptuses were 95.14% and 99.32%, which were sufficient for estimating genomic breeding value. In Experiment 2, the rates of dead cells in elongating conceptuses cryopreserved by slow freezing were comparable to those in fresh elongating conceptuses. In addition, we obtained healthy calves by the transfer of elongating conceptuses cryopreserved by slow freezing. Our findings indicate that the elongating conceptus transfer technology enables preimplantation genomic selection in cattle based on SNP chip analysis. Further studies on the optimization of cryopreservation methods for elongating conceptuses are required for practical application of the selection system.

Original languageEnglish
Pages (from-to)497-504
Number of pages8
JournalJournal of Reproduction and Development
Volume63
Issue number5
DOIs
Publication statusPublished - 2017

Fingerprint

conceptus
marker-assisted selection
calves
cattle
single nucleotide polymorphism
genotyping
cryopreservation
biopsy
freezing
technology transfer
genome
zona pellucida
sex determination analysis
cells
breeding value
methodology
genetic improvement
embryo (animal)
hatching
genomics

Keywords

  • Cattle
  • Cryopreservation
  • Elongating conceptus
  • Embryo transfer
  • Preimplantation genomic selection

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Production of calves by the transfer of cryopreserved bovine elongating conceptuses and possible application for preimplantation genomic selection. / Fujii, Takashi; Hirayama, Hiroki; Naito, Akira; Kashima, Masashi; Sakai, Hitomi; Fukuda, Shigeo; Yoshino, Hitomi; Moriyasu, Satoru; Kageyama, Soichi; Sugimoto, Yoshikazu; Matsuyama, Shuichi; Hayakawa, Hiroyuki; Kimura, Koji.

In: Journal of Reproduction and Development, Vol. 63, No. 5, 2017, p. 497-504.

Research output: Contribution to journalArticle

Fujii, T, Hirayama, H, Naito, A, Kashima, M, Sakai, H, Fukuda, S, Yoshino, H, Moriyasu, S, Kageyama, S, Sugimoto, Y, Matsuyama, S, Hayakawa, H & Kimura, K 2017, 'Production of calves by the transfer of cryopreserved bovine elongating conceptuses and possible application for preimplantation genomic selection', Journal of Reproduction and Development, vol. 63, no. 5, pp. 497-504. https://doi.org/10.1262/jrd.2017-025
Fujii, Takashi ; Hirayama, Hiroki ; Naito, Akira ; Kashima, Masashi ; Sakai, Hitomi ; Fukuda, Shigeo ; Yoshino, Hitomi ; Moriyasu, Satoru ; Kageyama, Soichi ; Sugimoto, Yoshikazu ; Matsuyama, Shuichi ; Hayakawa, Hiroyuki ; Kimura, Koji. / Production of calves by the transfer of cryopreserved bovine elongating conceptuses and possible application for preimplantation genomic selection. In: Journal of Reproduction and Development. 2017 ; Vol. 63, No. 5. pp. 497-504.
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