Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB)

Takayuki Otani, Toshihiro Hashizume, Tadahiro Nagaoka, Tomoko Fukuda, Careen K. Tang, David S. Salomon, Masaharu Seno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to approximately 50% of the conventional Fc-fusion design without an Fc domain of IgG. The refolded dimers could be easily analyzed and characterized by SDS-PAGE. Hinge-tagged soluble ErbBs demonstrated significant affinity for betacellulin and heregulin. The IgG hinge-tag should be a simple method to design soluble dimers that would be useful for high throughput screening of ligands, antagonists or derivatives.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalBiotechnology Letters
Volume32
Issue number3
DOIs
Publication statusPublished - Feb 1 2010

Keywords

  • Epidermal growth factor receptors
  • ErbB
  • Extracellular domain
  • Hinge region
  • Homodimer
  • Soluble receptor

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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