Production of bioactive triterpenes by Eriobotrya japonica calli

Shoko Taniguchi, Yoko Imayoshi, Eri Kobayashi, Yoshie Takamatsu, Hideyuki Ito, Tsutomu Hatano, Hiroshi Sakagami, Harukuni Tokuda, Hoyoku Nishino, Daigo Sugita, Susumu Shimura, Takashi Yoshida

Research output: Contribution to journalArticlepeer-review

270 Citations (Scopus)


Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2α-hydoxyursolic acid, maslinic acid, tormentic acid, 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2α, 19α-Dihydroxy-3-oxo-urs- 12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
Issue number3
Publication statusPublished - Feb 20 2002


  • Callus tissue culture
  • Epstein-Barr virus
  • Eriobotrya japonica
  • Rosaceae
  • Triterpene
  • Two-stage carcinogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture


Dive into the research topics of 'Production of bioactive triterpenes by Eriobotrya japonica calli'. Together they form a unique fingerprint.

Cite this