Abstract
Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2α-hydoxyursolic acid, maslinic acid, tormentic acid, 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2α, 19α-Dihydroxy-3-oxo-urs- 12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.
| Original language | English |
|---|---|
| Pages (from-to) | 315-323 |
| Number of pages | 9 |
| Journal | Phytochemistry |
| Volume | 59 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Feb 20 2002 |
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Keywords
- Callus tissue culture
- Epstein-Barr virus
- Eriobotrya japonica
- Rosaceae
- Triterpene
- Two-stage carcinogenesis
ASJC Scopus subject areas
- Plant Science
- Biochemistry
- Molecular Biology
- Organic Chemistry
- Drug Discovery
Cite this
Production of bioactive triterpenes by Eriobotrya japonica calli. / Taniguchi, Shoko; Imayoshi, Yoko; Kobayashi, Eri; Takamatsu, Yoshie; Ito, Hideyuki; Hatano, Tsutomu; Sakagami, Hiroshi; Tokuda, Harukuni; Nishino, Hoyoku; Sugita, Daigo; Shimura, Susumu; Yoshida, Takashi.
In: Phytochemistry, Vol. 59, No. 3, 20.02.2002, p. 315-323.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of bioactive triterpenes by Eriobotrya japonica calli
AU - Taniguchi, Shoko
AU - Imayoshi, Yoko
AU - Kobayashi, Eri
AU - Takamatsu, Yoshie
AU - Ito, Hideyuki
AU - Hatano, Tsutomu
AU - Sakagami, Hiroshi
AU - Tokuda, Harukuni
AU - Nishino, Hoyoku
AU - Sugita, Daigo
AU - Shimura, Susumu
AU - Yoshida, Takashi
PY - 2002/2/20
Y1 - 2002/2/20
N2 - Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2α-hydoxyursolic acid, maslinic acid, tormentic acid, 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2α, 19α-Dihydroxy-3-oxo-urs- 12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.
AB - Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2α-hydoxyursolic acid, maslinic acid, tormentic acid, 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2α, 19α-Dihydroxy-3-oxo-urs- 12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.
KW - Callus tissue culture
KW - Epstein-Barr virus
KW - Eriobotrya japonica
KW - Rosaceae
KW - Triterpene
KW - Two-stage carcinogenesis
UR - http://www.scopus.com/inward/record.url?scp=0037138276&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037138276&partnerID=8YFLogxK
U2 - 10.1016/S0031-9422(01)00455-1
DO - 10.1016/S0031-9422(01)00455-1
M3 - Article
C2 - 11830140
AN - SCOPUS:0037138276
VL - 59
SP - 315
EP - 323
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
IS - 3
ER -