Production of bioactive triterpenes by Eriobotrya japonica calli

Shoko Taniguchi; Yoko Imayoshi; Eri Kobayashi; Yoshie Takamatsu; Hideyuki Ito; Tsutomu Hatano; Hiroshi Sakagami; Harukuni Tokuda; Hoyoku Nishino; Daigo Sugita; Susumu Shimura; Takashi Yoshida

doi:10.1016/S0031-9422(01)00455-1

Production of bioactive triterpenes by Eriobotrya japonica calli

Shoko Taniguchi, Yoko Imayoshi, Eri Kobayashi, Yoshie Takamatsu, Hideyuki Ito, Tsutomu Hatano, Hiroshi Sakagami, Harukuni Tokuda, Hoyoku Nishino, Daigo Sugita, Susumu Shimura, Takashi Yoshida

Research output: Contribution to journal › Article › peer-review

260 Citations (Scopus)

Abstract

Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2α-hydoxyursolic acid, maslinic acid, tormentic acid, 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2α, 19α-Dihydroxy-3-oxo-urs- 12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.

Original language	English
Pages (from-to)	315-323
Number of pages	9
Journal	Phytochemistry
Volume	59
Issue number	3
DOIs	https://doi.org/10.1016/S0031-9422(01)00455-1
Publication status	Published - Feb 20 2002

Keywords

Callus tissue culture
Epstein-Barr virus
Eriobotrya japonica
Rosaceae
Triterpene
Two-stage carcinogenesis

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0031-9422(01)00455-1

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Production of bioactive triterpenes by Eriobotrya japonica calli. / Taniguchi, Shoko; Imayoshi, Yoko; Kobayashi, Eri; Takamatsu, Yoshie; Ito, Hideyuki; Hatano, Tsutomu; Sakagami, Hiroshi; Tokuda, Harukuni; Nishino, Hoyoku; Sugita, Daigo; Shimura, Susumu; Yoshida, Takashi.

In: Phytochemistry, Vol. 59, No. 3, 20.02.2002, p. 315-323.

Research output: Contribution to journal › Article › peer-review

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title = "Production of bioactive triterpenes by Eriobotrya japonica calli",

abstract = "Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2α-hydoxyursolic acid, maslinic acid, tormentic acid, 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2α, 19α-Dihydroxy-3-oxo-urs- 12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.",

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AU - Ito, Hideyuki

AU - Hatano, Tsutomu

AU - Sakagami, Hiroshi

AU - Tokuda, Harukuni

AU - Nishino, Hoyoku

AU - Sugita, Daigo

AU - Shimura, Susumu

AU - Yoshida, Takashi

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AB - Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2α-hydoxyursolic acid, maslinic acid, tormentic acid, 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2α, 19α-Dihydroxy-3-oxo-urs- 12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.

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ER -

Production of bioactive triterpenes by Eriobotrya japonica calli

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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