Abstract
Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2α-hydoxyursolic acid, maslinic acid, tormentic acid, 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2α, 19α-Dihydroxy-3-oxo-urs- 12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.
| Original language | English |
|---|---|
| Pages (from-to) | 315-323 |
| Number of pages | 9 |
| Journal | Phytochemistry |
| Volume | 59 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Feb 20 2002 |
Keywords
- Callus tissue culture
- Epstein-Barr virus
- Eriobotrya japonica
- Rosaceae
- Triterpene
- Two-stage carcinogenesis
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Plant Science
- Horticulture
