Production of benzoylformic acid from phenylglycine by saccharomycopsis lipolytica

Hiroshi Kanzaki, Atsuhiko Isobe, Yoshikazu Izumi, Hideaki Yamada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Microbial production of benzoylformic acid (BF), which can be used as a substrate of enzymatic synthesis of (R)-(—)-mandelic acid, was investigated. Among 145 strains of yeasts and actinomycetes, Saccharomycopsis lipolytica (IAM 4964) was the best producer of BF from dl-phenylglycine (DL-PG). Culture conditions for BF production by the organism were optimized. When 0.2 % fructose as a carbon source and 0.7% Bacto-tryptone as a nitrogen source were used in the presence of 4% DL-PG, 14.5 mg/ml of BF was produced (about 37 % molar yield) in 4 days of cultivation. BF was synthesized from the l-form of PG, but not from the d-form. The BF was isolated from culture broth in a crystalline form and physicochemically identified.

Original languageEnglish
Pages (from-to)2101-2105
Number of pages5
JournalAgricultural and Biological Chemistry
Volume54
Issue number8
DOIs
Publication statusPublished - 1990

Fingerprint

Saccharomycopsis
acids
mandelic acid
Actinobacteria
Fructose
Yeast
phenylglyoxylic acid
fructose
Nitrogen
Carbon
Yeasts
yeasts
Crystalline materials
synthesis
carbon
organisms
nitrogen
Substrates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Production of benzoylformic acid from phenylglycine by saccharomycopsis lipolytica. / Kanzaki, Hiroshi; Isobe, Atsuhiko; Izumi, Yoshikazu; Yamada, Hideaki.

In: Agricultural and Biological Chemistry, Vol. 54, No. 8, 1990, p. 2101-2105.

Research output: Contribution to journalArticle

Kanzaki, Hiroshi ; Isobe, Atsuhiko ; Izumi, Yoshikazu ; Yamada, Hideaki. / Production of benzoylformic acid from phenylglycine by saccharomycopsis lipolytica. In: Agricultural and Biological Chemistry. 1990 ; Vol. 54, No. 8. pp. 2101-2105.
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