Production of bacteriocin by Leuconostoc mesenteroides 406 isolated from Mongolian fermented mare's milk, airag

Wulijideligen, Takayuki Asahina, Kazushi Hara, Kensuke Arakawa, Hiroyuki Nakano, Taku Miyamoto

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The purification and characterization of a bacteriocin produced by Leuconostoc mesenteroides strain 406 that was isolated from traditional Mongolian fermented mare's milk, airag, were carried out. Leuconostoc mesenteroides strain 406 was identified on the basis of its morphological and biochemical characteristics and carbohydrate fermentation profile and by API 50 CH kit and 16S ribosomal DNA analyses. The neutral-pH cell-free supernatant of this bacterium inhibited the growth of several lactic acid bacteria and food spoilage and pathogenic organisms, including Listeria monocytogenes and Clostridium botulinum. The bacteriocin was heat-stable and not sensitive to acid and alkaline conditions, but was sensitive to several proteolytic enzymes such as pepsin, pronase E, proteinase K, trypsin, and α-chymotrypsin, but not catalase. Optimum bacteriocin production (4000 activity units/mL) was achieved when the strain was cultured at 25°C for 24-36h in Man Rogosa Sharpe medium. The bacteriocin was partially purified by ammonium sulfate precipitation (80% saturation), dialysis (cut-off MW: 1000), and gel filtration chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the bacteriocin had a molecular weight of approximately 3.3kDa. To our knowledge, this is the first report of the isolation of a bacteriocin-producing Leuconostoc strain from airag. An application to fermented milks would be desired.

Original languageEnglish
Pages (from-to)704-711
Number of pages8
JournalAnimal Science Journal
Volume83
Issue number10
DOIs
Publication statusPublished - Oct 2012

Fingerprint

mare milk
Leuconostoc mesenteroides
Bacteriocins
bacteriocins
Milk
Complement Hemolytic Activity Assay
Clostridium botulinum
Bacteria
Leuconostoc
Endopeptidase K
food spoilage
Pronase
fermented milk
Pepsin A
Ammonium Sulfate
Listeria monocytogenes
chymotrypsin
pepsin
dialysis
Ribosomal DNA

Keywords

  • Airag
  • Bacteriocin
  • Leuconostoc mesenteroides 406
  • Traditional fermented milk

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Production of bacteriocin by Leuconostoc mesenteroides 406 isolated from Mongolian fermented mare's milk, airag. / Wulijideligen; Asahina, Takayuki; Hara, Kazushi; Arakawa, Kensuke; Nakano, Hiroyuki; Miyamoto, Taku.

In: Animal Science Journal, Vol. 83, No. 10, 10.2012, p. 704-711.

Research output: Contribution to journalArticle

Wulijideligen ; Asahina, Takayuki ; Hara, Kazushi ; Arakawa, Kensuke ; Nakano, Hiroyuki ; Miyamoto, Taku. / Production of bacteriocin by Leuconostoc mesenteroides 406 isolated from Mongolian fermented mare's milk, airag. In: Animal Science Journal. 2012 ; Vol. 83, No. 10. pp. 704-711.
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