Production of a Light-Gated Proton Channel by Replacing the Retinal Chromophore with Its Synthetic Vinylene Derivative

Riho Takayama, Akimasa Kaneko, Takashi Okitsu, Satoshi P. Tsunoda, Kazumi Shimono, Misao Mizuno, Keiichi Kojima, Takashi Tsukamoto, Hideki Kandori, Yasuhisa Mizutani, Akimori Wada, Yuki Sudo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rhodopsin is widely distributed in organisms as a membrane-embedded photoreceptor protein, consisting of the apoprotein opsin and vitamin-A aldehyde retinal, A1-retinal and A2-retinal being the natural chromophores. Modifications of opsin (e.g., by mutations) have provided insight into the molecular mechanism of the light-induced functions of rhodopsins as well as providing tools in chemical biology to control cellular activity by light. Instead of the apoprotein opsin, in this study, we focused on the retinal chromophore and synthesized three vinylene derivatives of A2-retinal. One of them, C(14)-vinylene A2-retinal (14V-A2), was successfully incorporated into the opsin of a light-driven proton pump archaerhodopsin-3 (AR3). Electrophysiological experiments revealed that the opsin of AR3 (archaeopsin3, AO3) with 14V-A2 functions as a light-gated proton channel. The engineered proton channel showed characteristic photochemical properties, which are significantly different from those of AR3. Thus, we successfully produced a proton channel by replacing the chromophore of AR3.

Original languageEnglish
Pages (from-to)2857-2862
Number of pages6
JournalJournal of Physical Chemistry Letters
Volume9
Issue number11
DOIs
Publication statusPublished - Jun 7 2018

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Opsins
Chromophores
chromophores
Protons
Derivatives
protons
Apoproteins
Rhodopsin
vitamins
photoreceptors
Vitamins
Retinaldehyde
mutations
Proton Pumps
Aldehydes
aldehydes
biology
organisms
Pumps
pumps

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Production of a Light-Gated Proton Channel by Replacing the Retinal Chromophore with Its Synthetic Vinylene Derivative. / Takayama, Riho; Kaneko, Akimasa; Okitsu, Takashi; Tsunoda, Satoshi P.; Shimono, Kazumi; Mizuno, Misao; Kojima, Keiichi; Tsukamoto, Takashi; Kandori, Hideki; Mizutani, Yasuhisa; Wada, Akimori; Sudo, Yuki.

In: Journal of Physical Chemistry Letters, Vol. 9, No. 11, 07.06.2018, p. 2857-2862.

Research output: Contribution to journalArticle

Takayama, R, Kaneko, A, Okitsu, T, Tsunoda, SP, Shimono, K, Mizuno, M, Kojima, K, Tsukamoto, T, Kandori, H, Mizutani, Y, Wada, A & Sudo, Y 2018, 'Production of a Light-Gated Proton Channel by Replacing the Retinal Chromophore with Its Synthetic Vinylene Derivative', Journal of Physical Chemistry Letters, vol. 9, no. 11, pp. 2857-2862. https://doi.org/10.1021/acs.jpclett.8b00879
Takayama, Riho ; Kaneko, Akimasa ; Okitsu, Takashi ; Tsunoda, Satoshi P. ; Shimono, Kazumi ; Mizuno, Misao ; Kojima, Keiichi ; Tsukamoto, Takashi ; Kandori, Hideki ; Mizutani, Yasuhisa ; Wada, Akimori ; Sudo, Yuki. / Production of a Light-Gated Proton Channel by Replacing the Retinal Chromophore with Its Synthetic Vinylene Derivative. In: Journal of Physical Chemistry Letters. 2018 ; Vol. 9, No. 11. pp. 2857-2862.
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