Production of a bacteriocin-like inhibitory substance by Leuconostoc mesenteroides subsp. dextranicum 213M0 isolated from Mongolian fermented mare milk, airag

Kensuke Arakawa, Saki Yoshida, Hiroki Aikawa, Chihiro Hano, Tsognemekh Bolormaa, Sedkhuu Burenjargal, Taku Miyamoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Strain 213M0 was selected with productivity of a bacteriocin-like inhibitory substance (BLIS) among 235 strains of lactic acid bacteria (LAB) isolated from Mongolian fermented milk 'airag'. Strain 213M0 was species-identified as Leuconostoc mesenteroides subsp. dextranicum by morphological observation, carbohydrate fermentation profiling and sequencing the 16S rRNA gene. Incubation temperature proper to produce the BLIS was 25°C rather than 30 and 37°C, and the production actively proceeded during the exponential growth phase of the producer cells. Antibacterial effect of BLIS 213M0 was limited to all nine strains of Listeria sp. bacteria and seven strains of LAB cocci among 53 tested strains, which corresponds to a typical feature of the class IIa pediocin-like bacteriocins. BLIS 213M0 was not inactivated in every broad pH range solution (pH 2.0-11.0), and was stable against storage at 25°C for 1 week and heating at 121°C for 15 min under pH 4.5. Peptide frame of BLIS 213M0 was confirmed by inactivation with some peptidases, and then its molecular weight was estimated to be 2.6-3.0 kDa using an in situ activity assay following sodium dodecyl sulfate polyacrylamide gel electrophoresis. The estimated size was different from the other Leuconostoc bacteriocins already reported. These results suggest that BLIS 213M0 would be a novel listericidal bacteriocin.

Original languageEnglish
Pages (from-to)449-456
Number of pages8
JournalAnimal Science Journal
Volume87
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Leuconostoc mesenteroides subsp. dextranicum
mare milk
Bacteriocins
bacteriocins
Milk
Bacteria
lactic acid bacteria
Lactic Acid
pediocins
Leuconostoc
Leuconostoc mesenteroides
Listeria
Coccus
fermented milk
peptidases
rRNA Genes
Sodium Dodecyl Sulfate
Heating
Fermentation
polyacrylamide gel electrophoresis

Keywords

  • Airag
  • Bacteriocin
  • Lactic acid bacteria
  • Leuconostoc mesenteroides
  • Listericidal activity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Production of a bacteriocin-like inhibitory substance by Leuconostoc mesenteroides subsp. dextranicum 213M0 isolated from Mongolian fermented mare milk, airag. / Arakawa, Kensuke; Yoshida, Saki; Aikawa, Hiroki; Hano, Chihiro; Bolormaa, Tsognemekh; Burenjargal, Sedkhuu; Miyamoto, Taku.

In: Animal Science Journal, Vol. 87, No. 3, 01.03.2016, p. 449-456.

Research output: Contribution to journalArticle

Arakawa, Kensuke ; Yoshida, Saki ; Aikawa, Hiroki ; Hano, Chihiro ; Bolormaa, Tsognemekh ; Burenjargal, Sedkhuu ; Miyamoto, Taku. / Production of a bacteriocin-like inhibitory substance by Leuconostoc mesenteroides subsp. dextranicum 213M0 isolated from Mongolian fermented mare milk, airag. In: Animal Science Journal. 2016 ; Vol. 87, No. 3. pp. 449-456.
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