Production and partial properties of alginase from newly isolated Flavobacterium sp. LXA

Qing Da An, Gong Liang Zhang, Hai Tao Wu, Zhen Cheng Zhang, Wen Gong, Yan Liu, Xian Zhen Li, Yoshiyuki Murata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Alginase was produced as an extracellular alginate-degrading enzyme by a newly isolated Flavobacterium sp. LXA. The maximal alginase activity was obtained when strain LXA was cultured in the medium with alginate as carbon source, whereas some other carbohydrates including mannose, maltose, glucose, and fructose supported only a little alginase production. The addition of nitrogen sources was not necessary for alginase production by strain LXA grown in the mineral solution with alginate as carbon source. The endolytic mode on the molecule alginate was supported by the better correlation between the viscosity loss and the reducing group released in the process of alginate degradation. The relationship between the formation of reducing terminus and the increase in OD235 suggested that alginase was an eliminase cleaving alginate to form an unsaturated uronate residue absorbable at 235 nm and a reducing group at 1:1 ratio.

Original languageEnglish
Pages (from-to)842-847
Number of pages6
JournalProcess Biochemistry
Volume43
Issue number8
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Flavobacterium
Alginate
Carbon
Maltose
Fructose
Carbohydrates
Mannose
Viscosity
Minerals
Glucose
poly(beta-D-mannuronate) lyase
alginic acid
Nitrogen
Enzymes
Degradation
Molecules

Keywords

  • Alginase
  • Alginate
  • Alginate degradation
  • Flavobacterium
  • Oligosaccharide

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Engineering (miscellaneous)
  • Industrial and Manufacturing Engineering

Cite this

Production and partial properties of alginase from newly isolated Flavobacterium sp. LXA. / An, Qing Da; Zhang, Gong Liang; Wu, Hai Tao; Zhang, Zhen Cheng; Gong, Wen; Liu, Yan; Li, Xian Zhen; Murata, Yoshiyuki.

In: Process Biochemistry, Vol. 43, No. 8, 08.2008, p. 842-847.

Research output: Contribution to journalArticle

An, Qing Da ; Zhang, Gong Liang ; Wu, Hai Tao ; Zhang, Zhen Cheng ; Gong, Wen ; Liu, Yan ; Li, Xian Zhen ; Murata, Yoshiyuki. / Production and partial properties of alginase from newly isolated Flavobacterium sp. LXA. In: Process Biochemistry. 2008 ; Vol. 43, No. 8. pp. 842-847.
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AU - Liu, Yan

AU - Li, Xian Zhen

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