Production and characterization of recombinant vitellogenesis-inhibiting hormone from the American lobster Homarus americanus

Tsuyoshi Ohira, Takuji Okumura, Michio Suzuki, Yosuke Yajima, Naoaki Tsutsui, Marcy N. Wilder, Hiromichi Nagasawa

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Recombinant peptides related to vitellogenesis-inhibiting hormone (VIH) of the American lobster Homarus americanus were expressed in bacterial cells, and then purified after being allowed to refold. Biological activities of the recombinant VIHs having an amidated C-terminus (rHoa-VIH-amide) and a free carboxyl-terminus (rHoa-VIH-OH) were examined using an ovarian fragment incubation system derived from the kuruma prawn, Marsupenaeus japonicus. The rHoa-VIH-amide significantly reduced vitellogenin mRNA levels in the ovary, while rHoa-VIH-OH had no effect. This is the first report that describes the production of a crustacean VIH having biological activity and the importance of the C-terminal amidation for its vitellogenesis-inhibiting activity.

Original languageEnglish
Pages (from-to)1251-1258
Number of pages8
JournalPeptides
Volume27
Issue number6
DOIs
Publication statusPublished - Jun 1 2006

Keywords

  • American lobster
  • Carboxyl-terminal amide
  • Homarus americanus
  • Marsupenaeus japonicus
  • Vitellogenesis-inhibiting hormone

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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    Ohira, T., Okumura, T., Suzuki, M., Yajima, Y., Tsutsui, N., Wilder, M. N., & Nagasawa, H. (2006). Production and characterization of recombinant vitellogenesis-inhibiting hormone from the American lobster Homarus americanus. Peptides, 27(6), 1251-1258. https://doi.org/10.1016/j.peptides.2005.10.019