Abstract
Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].
| Original language | English |
|---|---|
| Pages (from-to) | 347-351 |
| Number of pages | 5 |
| Journal | Journal of the National Cancer Institute |
| Volume | 81 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 1 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Physiology (medical)
- Radiology Nuclear Medicine and imaging
- Oncology
- Cancer Research
Cite this
Production and characterization of human glioma cell-derived monocyte chemotactic factor. / Kuratsu, Jun ichi; Leonard, Edward J.; Yoshimura, Teizo.
In: Journal of the National Cancer Institute, Vol. 81, No. 5, 01.03.1989, p. 347-351.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production and characterization of human glioma cell-derived monocyte chemotactic factor
AU - Kuratsu, Jun ichi
AU - Leonard, Edward J.
AU - Yoshimura, Teizo
PY - 1989/3/1
Y1 - 1989/3/1
N2 - Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].
AB - Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].
UR - http://www.scopus.com/inward/record.url?scp=0024595493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024595493&partnerID=8YFLogxK
U2 - 10.1093/jnci/81.5.347
DO - 10.1093/jnci/81.5.347
M3 - Article
C2 - 2915371
AN - SCOPUS:0024595493
VL - 81
SP - 347
EP - 351
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 5
ER -