TY - JOUR
T1 - Production and characterization of human glioma cell-derived monocyte chemotactic factor
AU - Kuratsu, Jun ichi
AU - Leonard, Edward J.
AU - Yoshimura, Teizo
N1 - Funding Information:
Glioma is one of the brain tumors in which macrophage infiltration is frequently observed (12-14). Although the rel- Received September 23, 1988; revised December 1, 1988; accepted December 3, 1988. Dr. Yoshimura was supported by the Japanese Overseas Cancer Fellowship of the Foundation for Promotion of Cancer Research. • J. Kuratsu, Department of Neurosurgery, Kumamoto University Medical School, Kumamoto 860, Japan. E. J. Leonard, T. Yoshimura, Immunopathology Section, Laboratory of Immunobiology, National Cancer Institute, Frederick, MD. We thank Dr. Y. Gillespie (University of Alabama, Birmingham, AL) for glioma cell lines. *Correspondence to: Dr. Teizo Yoshimura, Immunopathology Section, Laboratory of Immunobiology, National Cancer Institute, Frederick, MD 21701.
PY - 1989/3/1
Y1 - 1989/3/1
N2 - Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].
AB - Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].
UR - http://www.scopus.com/inward/record.url?scp=0024595493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024595493&partnerID=8YFLogxK
U2 - 10.1093/jnci/81.5.347
DO - 10.1093/jnci/81.5.347
M3 - Article
C2 - 2915371
AN - SCOPUS:0024595493
VL - 81
SP - 347
EP - 351
JO - Cancer chemotherapy reports. Part 1
JF - Cancer chemotherapy reports. Part 1
SN - 0027-8874
IS - 5
ER -
