Production and characterization of human glioma cell-derived monocyte chemotactic factor

Jun ichi Kuratsu, Edward J. Leonard, Teizo Yoshimura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].

Original languageEnglish
Pages (from-to)347-351
Number of pages5
JournalJournal of the National Cancer Institute
Volume81
Issue number5
DOIs
Publication statusPublished - Mar 1 1989
Externally publishedYes

Fingerprint

Monocytes
Chemotactic Factors
Glioma
Tumors
Cell
Molecular mass
Cell culture
Infiltration
Cells
Fluids
Tumor
Inflammation Mediators
Neoplasms
Inflammation
Confluence
Mediator
Line
Isoelectric Point
Human
Factors

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Oncology
  • Cancer Research

Cite this

Production and characterization of human glioma cell-derived monocyte chemotactic factor. / Kuratsu, Jun ichi; Leonard, Edward J.; Yoshimura, Teizo.

In: Journal of the National Cancer Institute, Vol. 81, No. 5, 01.03.1989, p. 347-351.

Research output: Contribution to journalArticle

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