Production and characterization of human glioma cell-derived monocyte chemotactic factor

Jun ichi Kuratsu; Edward J. Leonard; Teizo Yoshimura

doi:10.1093/jnci/81.5.347

Production and characterization of human glioma cell-derived monocyte chemotactic factor

Jun ichi Kuratsu, Edward J. Leonard, Teizo Yoshimura

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].

Original language	English
Pages (from-to)	347-351
Number of pages	5
Journal	Journal of the National Cancer Institute
Volume	81
Issue number	5
DOIs	https://doi.org/10.1093/jnci/81.5.347
Publication status	Published - Mar 1 1989
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Production and characterization of human glioma cell-derived monocyte chemotactic factor",

abstract = "Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].",

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AB - Since infiltration of monocytes into tumors may be mediated by tumor-derived chemoattractants, we characterized the monocyte-chemotactic activity (MCA) produced by glioma cell lines. The amount of MCA in the culture fluid of five lines tested differed by a factor of 25. U-105MG, the best producer, was selected for further study. After cells reached confluence and the medium was changed, MCA was detected by day 3 and remained at comparable levels on days 4 and 5. The molecular mass of MCA was approximately 17 kilodaltons, and the estimated isoelectric point ranged between pI 7 and pI 9. Because of the high constitutive production of MCA by U-105MG, sufficient material can be obtained for complete chemical characterization of this mediator of inflammation. [J Natl Cancer Inst 81:347-351, 1989].

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