Production and characterization of a complete set of wheat-wild barley (Hordeum vulgare ssp. spontaneum) chromosome addition lines

Shin Taketa, Kazuyoshi Takeda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A wild barley, Hordeum vulgare L. ssp. spontaneum (C. Koch.) Thell. (syn. H. spontaneum C. Koch.) is considered to be the progenitor of cultivated barley (H. vulgare L. ssp. vulgare). A complete set of Triticum aestivum L. cv. 'Shinchunaga'-H. spontaneum OUH602 whole chromosome addition lines, and 5 telosomic addition lines were developed. Chromosome constitutions of the addition lines were confirmed by C-banding and genomic in situ hybridization. Addition lines for the entire 1H chromosome and its long arm are only available as monosomic and monotelosomic additions, respectively, because of sterility. All other lines were recovered as disomic or ditelosomic additions. Self-sterile monosomic 1H and monotelosomic 1HL addition lines can be reproduced by backcrossing the plants having simultaneous addition of the long arm of barley chromosome 6H, because the plants with such chromosome constitutions recover partial female fertility. In general, the phenotypic alternations observed in each of the wheat-H. spontaneum OUH602 addition lines were similar to those previously noted in the corresponding lines of the 'Chinese Spring' wheat-'Betzes' barley addition series and the primary trisomic series of the H. spontaneum accession OUH602. Monotelosomic 1HL addition and ditelosomic 1HS addition are especially valuable for unequivocal chromosome arm localization of barley genes and markers clustered around the centromeric region of 1H. The utility of the wheat-H. spontaneum addition lines is discussed.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalBreeding Science
Volume51
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

chromosome addition
Hordeum
Hordeum vulgare
Triticum
Chromosomes
barley
wheat
chromosomes
monosomics
Constitution and Bylaws
Hordeum vulgare subsp. spontaneum
disomics
trisomics
female fertility
backcrossing
chromosome banding
spring wheat
in situ hybridization
Inbreeding
Triticum aestivum

Keywords

  • Addition line
  • Barley
  • C-banding
  • Chromosome
  • Genomic in situ hybridization
  • H. spontaneum
  • Wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Production and characterization of a complete set of wheat-wild barley (Hordeum vulgare ssp. spontaneum) chromosome addition lines. / Taketa, Shin; Takeda, Kazuyoshi.

In: Breeding Science, Vol. 51, No. 3, 2001, p. 199-206.

Research output: Contribution to journalArticle

@article{a76b9cd71d054ee7bc57d47ccd8c98d5,
title = "Production and characterization of a complete set of wheat-wild barley (Hordeum vulgare ssp. spontaneum) chromosome addition lines",
abstract = "A wild barley, Hordeum vulgare L. ssp. spontaneum (C. Koch.) Thell. (syn. H. spontaneum C. Koch.) is considered to be the progenitor of cultivated barley (H. vulgare L. ssp. vulgare). A complete set of Triticum aestivum L. cv. 'Shinchunaga'-H. spontaneum OUH602 whole chromosome addition lines, and 5 telosomic addition lines were developed. Chromosome constitutions of the addition lines were confirmed by C-banding and genomic in situ hybridization. Addition lines for the entire 1H chromosome and its long arm are only available as monosomic and monotelosomic additions, respectively, because of sterility. All other lines were recovered as disomic or ditelosomic additions. Self-sterile monosomic 1H and monotelosomic 1HL addition lines can be reproduced by backcrossing the plants having simultaneous addition of the long arm of barley chromosome 6H, because the plants with such chromosome constitutions recover partial female fertility. In general, the phenotypic alternations observed in each of the wheat-H. spontaneum OUH602 addition lines were similar to those previously noted in the corresponding lines of the 'Chinese Spring' wheat-'Betzes' barley addition series and the primary trisomic series of the H. spontaneum accession OUH602. Monotelosomic 1HL addition and ditelosomic 1HS addition are especially valuable for unequivocal chromosome arm localization of barley genes and markers clustered around the centromeric region of 1H. The utility of the wheat-H. spontaneum addition lines is discussed.",
keywords = "Addition line, Barley, C-banding, Chromosome, Genomic in situ hybridization, H. spontaneum, Wheat",
author = "Shin Taketa and Kazuyoshi Takeda",
year = "2001",
doi = "10.1270/jsbbs.51.199",
language = "English",
volume = "51",
pages = "199--206",
journal = "Breeding Science",
issn = "1344-7610",
publisher = "Japanese Society of Breeding",
number = "3",

}

TY - JOUR

T1 - Production and characterization of a complete set of wheat-wild barley (Hordeum vulgare ssp. spontaneum) chromosome addition lines

AU - Taketa, Shin

AU - Takeda, Kazuyoshi

PY - 2001

Y1 - 2001

N2 - A wild barley, Hordeum vulgare L. ssp. spontaneum (C. Koch.) Thell. (syn. H. spontaneum C. Koch.) is considered to be the progenitor of cultivated barley (H. vulgare L. ssp. vulgare). A complete set of Triticum aestivum L. cv. 'Shinchunaga'-H. spontaneum OUH602 whole chromosome addition lines, and 5 telosomic addition lines were developed. Chromosome constitutions of the addition lines were confirmed by C-banding and genomic in situ hybridization. Addition lines for the entire 1H chromosome and its long arm are only available as monosomic and monotelosomic additions, respectively, because of sterility. All other lines were recovered as disomic or ditelosomic additions. Self-sterile monosomic 1H and monotelosomic 1HL addition lines can be reproduced by backcrossing the plants having simultaneous addition of the long arm of barley chromosome 6H, because the plants with such chromosome constitutions recover partial female fertility. In general, the phenotypic alternations observed in each of the wheat-H. spontaneum OUH602 addition lines were similar to those previously noted in the corresponding lines of the 'Chinese Spring' wheat-'Betzes' barley addition series and the primary trisomic series of the H. spontaneum accession OUH602. Monotelosomic 1HL addition and ditelosomic 1HS addition are especially valuable for unequivocal chromosome arm localization of barley genes and markers clustered around the centromeric region of 1H. The utility of the wheat-H. spontaneum addition lines is discussed.

AB - A wild barley, Hordeum vulgare L. ssp. spontaneum (C. Koch.) Thell. (syn. H. spontaneum C. Koch.) is considered to be the progenitor of cultivated barley (H. vulgare L. ssp. vulgare). A complete set of Triticum aestivum L. cv. 'Shinchunaga'-H. spontaneum OUH602 whole chromosome addition lines, and 5 telosomic addition lines were developed. Chromosome constitutions of the addition lines were confirmed by C-banding and genomic in situ hybridization. Addition lines for the entire 1H chromosome and its long arm are only available as monosomic and monotelosomic additions, respectively, because of sterility. All other lines were recovered as disomic or ditelosomic additions. Self-sterile monosomic 1H and monotelosomic 1HL addition lines can be reproduced by backcrossing the plants having simultaneous addition of the long arm of barley chromosome 6H, because the plants with such chromosome constitutions recover partial female fertility. In general, the phenotypic alternations observed in each of the wheat-H. spontaneum OUH602 addition lines were similar to those previously noted in the corresponding lines of the 'Chinese Spring' wheat-'Betzes' barley addition series and the primary trisomic series of the H. spontaneum accession OUH602. Monotelosomic 1HL addition and ditelosomic 1HS addition are especially valuable for unequivocal chromosome arm localization of barley genes and markers clustered around the centromeric region of 1H. The utility of the wheat-H. spontaneum addition lines is discussed.

KW - Addition line

KW - Barley

KW - C-banding

KW - Chromosome

KW - Genomic in situ hybridization

KW - H. spontaneum

KW - Wheat

UR - http://www.scopus.com/inward/record.url?scp=0034814628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034814628&partnerID=8YFLogxK

U2 - 10.1270/jsbbs.51.199

DO - 10.1270/jsbbs.51.199

M3 - Article

VL - 51

SP - 199

EP - 206

JO - Breeding Science

JF - Breeding Science

SN - 1344-7610

IS - 3

ER -