TY - JOUR
T1 - Producing cadmium-free Indica rice by overexpressing OsHMA3
AU - Lu, Chenni
AU - Zhang, Lingxiao
AU - Tang, Zhong
AU - Huang, Xin Yuan
AU - Ma, Jian Feng
AU - Zhao, Fang Jie
N1 - Funding Information:
The study was supported by the Special Fund for Agro-scientific Research in the Public Interest (grant number 201403015 ), the Natural Science Foundation of China (grant no. 21661132001 ), the innovative research team development plan of the Ministry of Education of China (grant no. IRT_17R56 ) and the Fundamental Research Funds for the Central Universities (grant no. KYT201802 ).
Publisher Copyright:
© 2019 The Authors
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2019/5
Y1 - 2019/5
N2 - Background: Considerable proportions of rice grains produced in some areas in southern China contain high concentrations of cadmium (Cd), leading to unsafe levels of dietary Cd intake. Cultivars of Indica rice, widely grown in southern China, are particularly prone to high Cd accumulation in the grain. Effective methods are needed to decrease Cd accumulation in Indica rice. Methods: OsHMA3, encoding a tonoplast Cd transporter, was overexpressed in an elite Indica rice cultivar (Zhongjiazao 17) driven by CaMV 35S promoter. The effects on Cd translocation, accumulation and tolerance, as well as on the agronomic traits and micronutrient concentrations were evaluated. Results: OsHMA3 overexpression markedly decreased Cd translocation from roots to shoots and increased Cd tolerance. OsHMA3 overexpression decreased Cd concentrations in brown rice by 94–98%, to levels just above the detection limit, when rice plants were grown in two Cd-contaminated paddy soils. OsHMA3 overexpression generally had no significant effect on grain yield and the concentrations of the essential micronutrients including zinc, iron, copper and manganese in field trials. Conclusion: Overexpression of OsHMA3 is a highly effective method to reduce Cd accumulation in Indica rice, producing rice grains that were almost Cd free with little effect on grain yield or essential micronutrient concentrations.
AB - Background: Considerable proportions of rice grains produced in some areas in southern China contain high concentrations of cadmium (Cd), leading to unsafe levels of dietary Cd intake. Cultivars of Indica rice, widely grown in southern China, are particularly prone to high Cd accumulation in the grain. Effective methods are needed to decrease Cd accumulation in Indica rice. Methods: OsHMA3, encoding a tonoplast Cd transporter, was overexpressed in an elite Indica rice cultivar (Zhongjiazao 17) driven by CaMV 35S promoter. The effects on Cd translocation, accumulation and tolerance, as well as on the agronomic traits and micronutrient concentrations were evaluated. Results: OsHMA3 overexpression markedly decreased Cd translocation from roots to shoots and increased Cd tolerance. OsHMA3 overexpression decreased Cd concentrations in brown rice by 94–98%, to levels just above the detection limit, when rice plants were grown in two Cd-contaminated paddy soils. OsHMA3 overexpression generally had no significant effect on grain yield and the concentrations of the essential micronutrients including zinc, iron, copper and manganese in field trials. Conclusion: Overexpression of OsHMA3 is a highly effective method to reduce Cd accumulation in Indica rice, producing rice grains that were almost Cd free with little effect on grain yield or essential micronutrient concentrations.
KW - Cadmium
KW - Food safety
KW - Genetic modification
KW - OsHMA3
KW - Rice
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U2 - 10.1016/j.envint.2019.03.004
DO - 10.1016/j.envint.2019.03.004
M3 - Article
C2 - 30856449
AN - SCOPUS:85062396393
VL - 126
SP - 619
EP - 626
JO - Environmental International
JF - Environmental International
SN - 0160-4120
ER -