Procollagen II amino propeptide processing by ADAMTS-3. Insights on dermatosparaxis

Russell J. Fernandes, Satoshi Hirohata, J. Michael Engle, Alain Colige, Daniel H. Cohn, David R. Eyre, Suneel S. Apte

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Abstract

The amino and carboxyl propeptides of procollagens I and II are removed by specific enzymes as a prerequisite for fibril assembly. Null mutations in procollagen I N-propeptidase (ADAMTS-2) cause dermatosparaxis in cattle and the Ehlers-Danlos syndrome (dermatosparactic type) in humans by preventing proteolytic excision of the N-propeptide of procollagen I. We have found that procollagen II is processed normally in dermatosparactic nasal cartilage, suggesting the existence of another N-propeptidase(s). We investigated such a role for ADAMTS-3 in Swarm rat chondrosarcoma RCS-LTC cells, which fail to process the procollagen II N-propeptide. Stable transfection of RCS-LTC cells with bovine ADAMTS-2 or human ADAMTS-3 partially rescued the processing defect, suggesting that ADAMTS-3 has procollagen II N-propeptidase activity. Human skin and skin fibroblasts showed 30-fold higher mRNA levels of ADAMTS-2 than ADAMTS-3, whereas ADAMTS-3 mRNA was 5-fold higher than ADAMTS-2 mRNA in human cartilage. We propose that both ADAMTS-2 and ADAMTS-3 process procollagen II, but ADAMTS-3 is physiologically more relevant, given its preferred expression in cartilage. The findings provide an explanation for the sparing of cartilage in dermatosparaxis and, perhaps, for the relative sparing of some procollagen I-containing tissues.

Original languageEnglish
Pages (from-to)31502-31509
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number34
DOIs
Publication statusPublished - Aug 24 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fernandes, R. J., Hirohata, S., Engle, J. M., Colige, A., Cohn, D. H., Eyre, D. R., & Apte, S. S. (2001). Procollagen II amino propeptide processing by ADAMTS-3. Insights on dermatosparaxis. Journal of Biological Chemistry, 276(34), 31502-31509. https://doi.org/10.1074/jbc.M103466200