Processing of the hepatitis C virus precursor protein

K. Shimotohno, Y. Tanji, Y. Hirowatari, Y. Komoda, N. Kato, M. Hijikata

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Proteins of hepatitis C virus (HCV) are produced from a polyprotein precursor by post-translational processing. Production of HCV proteins was analyzed with in vitro translation, as well as plasmid-based transient gene expression, in mammalian cell lines. A minimum of three different processing pathways yielded at least 10 viral proteins from the polyprotein precursor. One pathway depended on signal protease processing, and the other two pathways utilized viral proteinases. The signal peptidase cleaved the viral structural proteins, and two viral activities broke up the viral nonstructural proteins. With staggered cleavages, the signal peptidase produced two E2 products from the E2 region, gp70 type A and type B, differing in the C-terminal structure. Two viral proteinases partially overlapped in the N-terminal region of NS3; the functional amino acid residues required for those two activities differed. Most of the processed viral proteins bound together; some of the associated proteins were membrane bound.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalJournal of Hepatology, Supplement
Volume22
Issue number1
Publication statusPublished - Jan 1 1995
Externally publishedYes

Keywords

  • Hepatitis C virus proteins
  • Proteinase
  • Proteolytic cleavage
  • Signal peptidase
  • Translation

ASJC Scopus subject areas

  • Hepatology

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  • Cite this

    Shimotohno, K., Tanji, Y., Hirowatari, Y., Komoda, Y., Kato, N., & Hijikata, M. (1995). Processing of the hepatitis C virus precursor protein. Journal of Hepatology, Supplement, 22(1), 87-92.