Processing and activation of recombinant mouse mastocytoma histidine decarboxylase in the particulate fraction of Sf9 cells by porcine pancreatic elastase

Satoshi Tanaka, Tetsuya Fukui, Jun Yamamoto, Yoh ichiro Shima, Toshiaki Kume, Makoto Ohgo, Atsushi Ichikawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Mature 53 kDa histidine decarboxylase (HDC) peptide is produced from a precursor 74 kDa peptide. The mechanism of specific cleavage by processing enzyme is unknown. Using the recombinant mouse 74 kDa HDC, we found that porcine pancreatic elastase specifically converted the inactive 74 kDa HDC to its active form of 53 kDa HDC.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1253
Issue number1
DOIs
Publication statusPublished - Nov 15 1995
Externally publishedYes

Fingerprint

Histidine Decarboxylase
Mastocytoma
Sf9 Cells
Pancreatic Elastase
Swine
Chemical activation
Processing
Peptides
Enzymes

Keywords

  • (Mouse)
  • (Porcine)
  • Histidine decarboxylase
  • Mastocytoma
  • Pancreatic elastase
  • Sf9 cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology

Cite this

Processing and activation of recombinant mouse mastocytoma histidine decarboxylase in the particulate fraction of Sf9 cells by porcine pancreatic elastase. / Tanaka, Satoshi; Fukui, Tetsuya; Yamamoto, Jun; Shima, Yoh ichiro; Kume, Toshiaki; Ohgo, Makoto; Ichikawa, Atsushi.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 1253, No. 1, 15.11.1995, p. 9-12.

Research output: Contribution to journalArticle

Tanaka, Satoshi ; Fukui, Tetsuya ; Yamamoto, Jun ; Shima, Yoh ichiro ; Kume, Toshiaki ; Ohgo, Makoto ; Ichikawa, Atsushi. / Processing and activation of recombinant mouse mastocytoma histidine decarboxylase in the particulate fraction of Sf9 cells by porcine pancreatic elastase. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1995 ; Vol. 1253, No. 1. pp. 9-12.
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