Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract

H. Musikasang, Akiwo Tani, A. H-kittikun, S. Maneerat

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

This study was conducted in order to evaluate the probiotic properties of lactic acid bacteria (LAB) isolated from intestinal tract of broilers and Thai indigenous chickens. The major properties, including the gastric juice and bile salts tolerance, starch, protein and lipid digesting capabilities, and the inhibition on certain pathogenic bacteria were investigated. Three-hundred and twenty-two and 226 LAB strains were isolated from ten broilers and eight Thai indigenous chickens, respectively. The gastrointestinal transit tolerance of these 548 isolates was determined by exposing washed cell suspension at 41°C to simulated gastric juice (pH 2.5) containing pepsin (3 mg ml-1), and to simulated small intestinal juice (pH 8.0) in the presence of pancreatin (1 mg ml-1) and 7% fresh chicken bile, mimicking the gastrointestinal environment. The survival of 20 isolates was found after passing through the gastrointestinal conditions. The survival rates of six strains; KT3L20, KT2CR5, KT10L22, KT5S19, KT4S13 and PM1L12 from the sequential study were 43.68, 37.56, 33.84, 32.89, 31.37 and 27.19%, respectively. Twelve isolates exhibited protein digestion on agar plate but no isolates showed the ability to digest starch and lipid. All 20 LAB showed the antimicrobial activity against Salmonella sp., Staphylococcus aureus and Escherichia coli except one strain which did not show the inhibitory activity toward E. coli. Accordingly, five isolates of selected LAB (KT2L24, KT3L20, KT4S13, KT3CE27 and KT8S16) can be classified as the best probiotics and were identified as Enterococcus faecalis, Enterococcus durans, Enterococcus faecium, Pediococcus pentosaceus, and Enterococcus faecium, respectively. The survival rate of microencapsulation of E. durans KT3L20 under simulated small intestine juice after sequential of simulated gastric juice was also investigated. An extrusion technique exhibited a higher survival rate than emulsion technique and free cell, respectively.

Original languageEnglish
Pages (from-to)1337-1345
Number of pages9
JournalWorld Journal of Microbiology and Biotechnology
Volume25
Issue number8
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Probiotics
Gastrointestinal Tract
Chickens
Lactic Acid
Gastric Juice
Bacteria
Enterococcus faecium
Starch
Pancreatin
Gastrointestinal Transit
Escherichia coli
Salt-Tolerance
Lipids
Drug Compounding
Enterococcus faecalis
Pepsin A
Enterococcus
Emulsions
Bile Acids and Salts
Bile

Keywords

  • Broiler
  • Chicken intestinal tract
  • Lactic acid bacteria
  • Probiotic
  • Thai indigenous chicken

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Cite this

Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract. / Musikasang, H.; Tani, Akiwo; H-kittikun, A.; Maneerat, S.

In: World Journal of Microbiology and Biotechnology, Vol. 25, No. 8, 07.2009, p. 1337-1345.

Research output: Contribution to journalArticle

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