Prion protein (PrP) is not involved in the pathogenesis of spongiform encephalopathy in zitter rats

Hiroshi Gomi, Toshio Ikeda, Tetsuo Kunieda, Shigeyoshi Itohara, Stanley B. Prusiner, Kazuya Yamanouchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In order to elucidate the relationship between the prion protein (PrP) structure and the development of spongiform encephalopathy in zitter rats, we analyzed the nucleotide sequences and restriction fragment length variation (RFLV) of the Prn gene encoding PrP in zitter rats and inbred SD/J rats as a control. Prn genes from two strains had identical nucleotide sequences in their coding sequences. Obvious RFLV on the locus was not detected in zitter rats by a Southern blot hybridization. Consistently, zitter rat brains express the normal cellular PrP (PrPC), but do not accumulate the protease-resistant modified isoform (PrPSC). These results indicate that PrP is not involved in the pathogenesis of spongiform encephalopathy in zitter rats.

Original languageEnglish
Pages (from-to)171-174
Number of pages4
JournalNeuroscience Letters
Volume166
Issue number2
DOIs
Publication statusPublished - Jan 31 1994
Externally publishedYes

Fingerprint

Brain Diseases
Southern Blotting
Genes
Prion Proteins
Protein Isoforms
Peptide Hydrolases
Brain

Keywords

  • Polymerase chain reaction
  • Prion protein
  • Prn
  • Spongiform encephalopathy
  • Zitter rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Prion protein (PrP) is not involved in the pathogenesis of spongiform encephalopathy in zitter rats. / Gomi, Hiroshi; Ikeda, Toshio; Kunieda, Tetsuo; Itohara, Shigeyoshi; Prusiner, Stanley B.; Yamanouchi, Kazuya.

In: Neuroscience Letters, Vol. 166, No. 2, 31.01.1994, p. 171-174.

Research output: Contribution to journalArticle

Gomi, Hiroshi ; Ikeda, Toshio ; Kunieda, Tetsuo ; Itohara, Shigeyoshi ; Prusiner, Stanley B. ; Yamanouchi, Kazuya. / Prion protein (PrP) is not involved in the pathogenesis of spongiform encephalopathy in zitter rats. In: Neuroscience Letters. 1994 ; Vol. 166, No. 2. pp. 171-174.
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