Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish

Tatsuya Sakamoto, Madoka Yoshiki, Hideya Takahashi, Masayuki Yoshida, Yukiko Ogino, Toshitaka Ikeuchi, Tomoya Nakamachi, Norifumi Konno, Kouhei Matsuda, Hirotaka Sakamoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

As in osmoregulation, mineralocorticoid signaling is implicated in the control of brain-behavior actions. Nevertheless, the understanding of this role is limited, partly due to the mortality of mineralocorticoid receptor (MR)-knockout (KO) mice due to impaired Na + reabsorption. In teleost fish, a distinct mineralocorticoid system has only been identified recently. Here, we generated a constitutive MR-KO medaka as the first adult-viable MR-KO animal, since MR expression is modest in osmoregulatory organs but high in the brain of adult medaka as for most teleosts. Hyper- and hypo-osmoregulation were normal in MR-KO medaka. When we studied the behavioral phenotypes based on the central MR localization, however, MR-KO medaka failed to track moving dots despite having an increase in acceleration of swimming. These findings reinforce previous results showing a minor role for mineralocorticoid signaling in fish osmoregulation, and provide the first convincing evidence that MR is required for normal locomotor activity in response to visual motion stimuli, but not for the recognition of these stimuli per se. We suggest that MR potentially integrates brain-behavioral and visual responses, which might be a conserved function of mineralocorticoid signaling through vertebrates. Importantly, this fish model allows for the possible identification of novel aspects of mineralocorticoid signaling.

Original languageEnglish
Article number37991
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Nov 29 2016

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Oryzias
Mineralocorticoid Receptors
Mineralocorticoids
Fishes
Osmoregulation
Brain
Behavior Control
Locomotion
Knockout Mice
Vertebrates
Phenotype

ASJC Scopus subject areas

  • General

Cite this

Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish. / Sakamoto, Tatsuya; Yoshiki, Madoka; Takahashi, Hideya; Yoshida, Masayuki; Ogino, Yukiko; Ikeuchi, Toshitaka; Nakamachi, Tomoya; Konno, Norifumi; Matsuda, Kouhei; Sakamoto, Hirotaka.

In: Scientific Reports, Vol. 6, 37991, 29.11.2016.

Research output: Contribution to journalArticle

Sakamoto, Tatsuya ; Yoshiki, Madoka ; Takahashi, Hideya ; Yoshida, Masayuki ; Ogino, Yukiko ; Ikeuchi, Toshitaka ; Nakamachi, Tomoya ; Konno, Norifumi ; Matsuda, Kouhei ; Sakamoto, Hirotaka. / Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish. In: Scientific Reports. 2016 ; Vol. 6.
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