Primary structures of N-linked oligosaccharides of momordin-a, a ribosome-inactivating protein from Momordica charantia seeds.

Yoshinobu Kimura, Yuji Minami, Toshihiko Tokuda, Shûhei Nakajima, Shigeaki Takagi, Gunki Funatsu

Research output: Contribution to journalArticle

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Abstract

The structures of the N-linked oligosaccharides of momordin-a, which is a ribosome-inactivating protein from the seeds of Momordica charantia, were analyzed. First, the N-linked oligosaccharides of this glycoprotein were liberated by hydrazinolysis. After N-acetylation, the reducing ends of the oligosaccharides were coupled with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and high performance liquid chromatography (HPLC) on an ODS-silica column. Three kinds of oligosaccharide fractions were separated by HPLC. The structure of each oligosaccharide isolated was analyzed by a combination of sugar component analysis, exoglycosidase digestion, another kind of HPLC using an amide-silica column, and 500-MHz 1H NMR spectroscopy. The structures of two main oligosaccharides were established to be: [Formula; see text] and [Formula; see text]. These two oligosaccharides were the first examples having xylose (or fucose) but no alpha-mannosyl linkage among the N-linked oligosaccharides of glycoproteins from both animal and plant origins.

Original languageEnglish
Pages (from-to)2031-2036
Number of pages6
JournalAgricultural and Biological Chemistry
Volume55
Issue number8
DOIs
Publication statusPublished - Aug 1991

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Momordica charantia
Ribosome Inactivating Proteins
ribosomes
Oligosaccharides
oligosaccharides
Seed
Seeds
seeds
High performance liquid chromatography
proteins
high performance liquid chromatography
High Pressure Liquid Chromatography
Silicon Dioxide
silica
glycoproteins
Glycoproteins
Acetylation
Fucose
fucose
momordin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Primary structures of N-linked oligosaccharides of momordin-a, a ribosome-inactivating protein from Momordica charantia seeds. / Kimura, Yoshinobu; Minami, Yuji; Tokuda, Toshihiko; Nakajima, Shûhei; Takagi, Shigeaki; Funatsu, Gunki.

In: Agricultural and Biological Chemistry, Vol. 55, No. 8, 08.1991, p. 2031-2036.

Research output: Contribution to journalArticle

Kimura, Yoshinobu ; Minami, Yuji ; Tokuda, Toshihiko ; Nakajima, Shûhei ; Takagi, Shigeaki ; Funatsu, Gunki. / Primary structures of N-linked oligosaccharides of momordin-a, a ribosome-inactivating protein from Momordica charantia seeds. In: Agricultural and Biological Chemistry. 1991 ; Vol. 55, No. 8. pp. 2031-2036.
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