Primary Structures of N-Linked Oligosaccharides of Momordin-a, a Ribosome-inactivating Protein from Momordica charantia Seeds

Yoshinobu Kimura, Yuji Minami, Toshihiko Tokuda, Shûhei Nakajima, Shigeaki Takagi, Gunki Funatsu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The structures of the N-linked oligosaccharides of momordin-a, which is a ribosome-inactivating protein from the seeds of Momordica charantia, were analyzed. First, the N-linked oligosaccharides of this glycoprotein were liberated by hydrazinolysis. After N-acetylation, the reducing ends of the oligosaccharides were coupled with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and high performance liquid chromatography (HPLC) on an ODS-silica column. Three kinds of oligosaccharide fractions were separated by HPLC. The structure of each oligosaccharide isolated was analyzed by a combination of sugar component analysis, exoglycosidase digestion, another kind of HPLC using an amide-silica column, and 500-MHz 1H NMR spectroscopy. The structures of two main oligosaccharides were established to be: Xyl1β2Manβ4GlcNAcβ4GlcNAc Fucα3 and Xylβ2Manβ4GlcNAcβ4GlcNAc These two oligosaccharides were the first examples having xylose (or fucose) but no α-mannosyl linkage among the N-linked oligosaccharides of glycoproteins from both animal and plant origins.

Original languageEnglish
Pages (from-to)2031-2036
Number of pages6
JournalAgricultural and Biological Chemistry
Volume55
Issue number8
DOIs
Publication statusPublished - Jan 1 1991

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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