Primary radical pair in the Photosystem II reaction centre

Yuichiro Takahashi, Örjan Hansson, Paul Mathis, Kimiyuki Satoh

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Primary Photosystem II (PS II) reactions have been studied by flash absorption spectroscopy with nanosecond resolution. In isolated reaction centers which are devoid of the 47- and 43-kDa chlorophyll-binding polypeptides, an absorption change (ΔA) is induced immediately by a flash. The difference spectrum (450-560 nm, 820 nm) indicates the formation of a radical pair (P-680+, Pheo-) decaying with t 1 2 = 40 ns at 120 K. At 140 ns after the flash the ΔA can be attributed to the triplet state of P-680 (3P-680) and of a carotenoid (3Car, λmax = 545 nm). 3Car is formed with a yield of ≈3% and it rises with t 1 2 ≈ 12 ns. At 276 K, the radical pair decays, with t 1 2 ≈ 32 ns. One radical pair is formed per 20 chlorophylls. The data are best interpreted if 3P-680 is formed as a product of radical pair recombination with a yield of 23% at 276 K (80% at 10 K) and if 3Car is formed only in a minority of pigment complexes. The ΔA at 820 nm disappear under conditions (addition of dithionite and methyl viologen, plus continuous illumination) designed to reduce pheophytin. The signals reappear after turning off the continuous light. More intact PS II particles (core complex) were also studied. At 820 nm, the data show that P-680+ is formed under oxidizing conditions and is re-reduced in the microsecond time range. Under reducing conditions the primary biradical decays with t 1 2 = 25 ns. 3P-680 is formed, with properties analogous to those in isolated reaction centers. 3Car is also formed (λmax = 535 nm). The results show that the PS II reaction center has strong functional analogies with the reaction center of purple bacteria. The behavior of the carotenoid is a remarkable exception. The primary biradical decays much more slowly than expected on the basis of fluorescence measurements.

Original languageEnglish
Pages (from-to)49-59
Number of pages11
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume893
Issue number1
DOIs
Publication statusPublished - Aug 12 1987

Fingerprint

Photosystem II Protein Complex
Carotenoids
Chlorophyll
Pheophytins
Dithionite
Proteobacteria
Paraquat
Lighting
Absorption spectroscopy
Pigments
Genetic Recombination
Spectrum Analysis
Fluorescence
Light
Peptides
P-680

Keywords

  • Carotenoid
  • Electron transfer
  • Pheophytin
  • Photosynthesis
  • Triplet state

ASJC Scopus subject areas

  • Biophysics

Cite this

Primary radical pair in the Photosystem II reaction centre. / Takahashi, Yuichiro; Hansson, Örjan; Mathis, Paul; Satoh, Kimiyuki.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 893, No. 1, 12.08.1987, p. 49-59.

Research output: Contribution to journalArticle

Takahashi, Yuichiro ; Hansson, Örjan ; Mathis, Paul ; Satoh, Kimiyuki. / Primary radical pair in the Photosystem II reaction centre. In: Biochimica et Biophysica Acta - Bioenergetics. 1987 ; Vol. 893, No. 1. pp. 49-59.
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AB - Primary Photosystem II (PS II) reactions have been studied by flash absorption spectroscopy with nanosecond resolution. In isolated reaction centers which are devoid of the 47- and 43-kDa chlorophyll-binding polypeptides, an absorption change (ΔA) is induced immediately by a flash. The difference spectrum (450-560 nm, 820 nm) indicates the formation of a radical pair (P-680+, Pheo-) decaying with t 1 2 = 40 ns at 120 K. At 140 ns after the flash the ΔA can be attributed to the triplet state of P-680 (3P-680) and of a carotenoid (3Car, λmax = 545 nm). 3Car is formed with a yield of ≈3% and it rises with t 1 2 ≈ 12 ns. At 276 K, the radical pair decays, with t 1 2 ≈ 32 ns. One radical pair is formed per 20 chlorophylls. The data are best interpreted if 3P-680 is formed as a product of radical pair recombination with a yield of 23% at 276 K (80% at 10 K) and if 3Car is formed only in a minority of pigment complexes. The ΔA at 820 nm disappear under conditions (addition of dithionite and methyl viologen, plus continuous illumination) designed to reduce pheophytin. The signals reappear after turning off the continuous light. More intact PS II particles (core complex) were also studied. At 820 nm, the data show that P-680+ is formed under oxidizing conditions and is re-reduced in the microsecond time range. Under reducing conditions the primary biradical decays with t 1 2 = 25 ns. 3P-680 is formed, with properties analogous to those in isolated reaction centers. 3Car is also formed (λmax = 535 nm). The results show that the PS II reaction center has strong functional analogies with the reaction center of purple bacteria. The behavior of the carotenoid is a remarkable exception. The primary biradical decays much more slowly than expected on the basis of fluorescence measurements.

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