Preventive effects of anthraquinone food pigments on the DNA damage induced by carcinogens in Drosophila

Eizo Takahashi, Timothy H. Marczylo, Toshiro Watanabe, Shiro Nagai, Hikoya Hayatsu, Tomoe Negishi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We have previously demonstrated the inhibitory effect of chlorophyllin, a green food additive, on the genotoxicities of various carcinogens in Drosophila. Recently, we reported that purpurin, a component of a red food additive produced from madder root (Rubia tinctorium), inhibits the bacterial mutagenicity of heterocyclic amines. In the present study, we examined antigenotoxic activities of various pigments that are either constituents of food or food additives, using Drosophila in vivo DNA repair assay. Third instar larvae of Drosophila were fed a mutagen with or without pigment. The resulting adult flies were monitored for their male (repair deficient)/female (repair proficient) ratios, which reflect the DNA damage. We tested a total of 20 pigments, which are mainly of plant origins, including flavonoids, carotenoids, anthocyanins, anthraquinones and β-diketone (curcumin)-derivatives, against the genotoxicities of eight carcinogens; IQ, MeIQx, AFB1, NDMA, 2-AAF, DMBA, 4NQO, and MNU. Four anthraquinone pigments (alizarin, purpurin, lac color, and cochineal extract) showed significant antigenotoxic activities. Alizarin and purpurin suppressed the DNA damage induced by IQ, MeIQx, AFB1, NDMA, 2-AAF, DMBA, and MNU. Lac color and cochineal extract showed inhibition against IQ, MeIQx, AFB1, 2-AAF and DMBA. In these inhibitions, suppression of metabolic enzymes may be involved. Since purpurin and alizarin suppressed the activity of MNU, a direct alkylating agent, there may also be a mechanism distinct from enzyme inhibitions in these anthraquinone-mediated suppressions of DNA damage.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume480-481
Publication statusPublished - Sep 1 2001

Fingerprint

Anthraquinones
2-Acetylaminofluorene
Carcinogens
9,10-Dimethyl-1,2-benzanthracene
Food Additives
DNA Damage
Drosophila
Food
Rubia
Color
Curcumin
Anthocyanins
Alkylating Agents
Mutagens
Enzymes
Carotenoids
Flavonoids
Diptera
DNA Repair
Amines

Keywords

  • Alizarin
  • Anthraquinone
  • Antigenotoxicity
  • Drosophila
  • Food pigment

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Molecular Biology

Cite this

Preventive effects of anthraquinone food pigments on the DNA damage induced by carcinogens in Drosophila. / Takahashi, Eizo; Marczylo, Timothy H.; Watanabe, Toshiro; Nagai, Shiro; Hayatsu, Hikoya; Negishi, Tomoe.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 480-481, 01.09.2001, p. 139-145.

Research output: Contribution to journalArticle

Takahashi, Eizo ; Marczylo, Timothy H. ; Watanabe, Toshiro ; Nagai, Shiro ; Hayatsu, Hikoya ; Negishi, Tomoe. / Preventive effects of anthraquinone food pigments on the DNA damage induced by carcinogens in Drosophila. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2001 ; Vol. 480-481. pp. 139-145.
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