Abstract
Collagen-induced arthritis (CIA) is an excellent model of rheumatoid arthritis (RA) in humans that is induced in DBA/1 mice immunized with bovine type II collagen (CII). Here, we report that the induction of CIA was effectively suppressed by oral administration of AZ-9, a purified polysaccharide with the average molecular weight of approximately 200 kDa that was produced by a soil bacterium, Klebsiella oxytoca. When AZ-9 was administered at 125-250 mg/kg/day orally for 9 consecutive days after immunization with CII followed by its administration every 3 days, resulted in a marked reduction of the incidence and the severity of CIA. The serum level of anti-CII IgG2a and the production of IFN-γ and IL-12 in the draining lymph node (LN) cells were significantly lower in AZ-9-administered mice than the untreated control. These findings suggest that orally administered AZ-9 suppressed CIA through attenuating a Th1-type response to CII. AZ-9 could be fragmented into smaller molecules (3-4 kDa) without losing its suppressive activity. (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 325-333 |
| Number of pages | 9 |
| Journal | Immunopharmacology |
| Volume | 49 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2000 |
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Keywords
- Anti-rheumatic agent
- Bacterial polysaccharide
- Collagen-induced arthritis
- Th1 cytokines
ASJC Scopus subject areas
- Pharmacology
Cite this
Prevention of collagen-induced arthritis in DBA/1 mice by oral administration of AZ-9, a bacterial polysaccharide from Klebsiella oxytoca. / Sugihara, Ryosuke; Yoshimura, Masayasu; Mori, Masaharu; Kanayama, Naoki; Hikida, Masaki; Ohmori, Hitoshi.
In: Immunopharmacology, Vol. 49, No. 3, 2000, p. 325-333.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prevention of collagen-induced arthritis in DBA/1 mice by oral administration of AZ-9, a bacterial polysaccharide from Klebsiella oxytoca
AU - Sugihara, Ryosuke
AU - Yoshimura, Masayasu
AU - Mori, Masaharu
AU - Kanayama, Naoki
AU - Hikida, Masaki
AU - Ohmori, Hitoshi
PY - 2000
Y1 - 2000
N2 - Collagen-induced arthritis (CIA) is an excellent model of rheumatoid arthritis (RA) in humans that is induced in DBA/1 mice immunized with bovine type II collagen (CII). Here, we report that the induction of CIA was effectively suppressed by oral administration of AZ-9, a purified polysaccharide with the average molecular weight of approximately 200 kDa that was produced by a soil bacterium, Klebsiella oxytoca. When AZ-9 was administered at 125-250 mg/kg/day orally for 9 consecutive days after immunization with CII followed by its administration every 3 days, resulted in a marked reduction of the incidence and the severity of CIA. The serum level of anti-CII IgG2a and the production of IFN-γ and IL-12 in the draining lymph node (LN) cells were significantly lower in AZ-9-administered mice than the untreated control. These findings suggest that orally administered AZ-9 suppressed CIA through attenuating a Th1-type response to CII. AZ-9 could be fragmented into smaller molecules (3-4 kDa) without losing its suppressive activity. (C) 2000 Elsevier Science B.V.
AB - Collagen-induced arthritis (CIA) is an excellent model of rheumatoid arthritis (RA) in humans that is induced in DBA/1 mice immunized with bovine type II collagen (CII). Here, we report that the induction of CIA was effectively suppressed by oral administration of AZ-9, a purified polysaccharide with the average molecular weight of approximately 200 kDa that was produced by a soil bacterium, Klebsiella oxytoca. When AZ-9 was administered at 125-250 mg/kg/day orally for 9 consecutive days after immunization with CII followed by its administration every 3 days, resulted in a marked reduction of the incidence and the severity of CIA. The serum level of anti-CII IgG2a and the production of IFN-γ and IL-12 in the draining lymph node (LN) cells were significantly lower in AZ-9-administered mice than the untreated control. These findings suggest that orally administered AZ-9 suppressed CIA through attenuating a Th1-type response to CII. AZ-9 could be fragmented into smaller molecules (3-4 kDa) without losing its suppressive activity. (C) 2000 Elsevier Science B.V.
KW - Anti-rheumatic agent
KW - Bacterial polysaccharide
KW - Collagen-induced arthritis
KW - Th1 cytokines
UR - http://www.scopus.com/inward/record.url?scp=0033829477&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033829477&partnerID=8YFLogxK
U2 - 10.1016/S0162-3109(00)00247-2
DO - 10.1016/S0162-3109(00)00247-2
M3 - Article
C2 - 10996030
AN - SCOPUS:0033829477
VL - 49
SP - 325
EP - 333
JO - Immunopharmacology
JF - Immunopharmacology
SN - 0162-3109
IS - 3
ER -