Prevention of collagen-induced arthritis in DBA/1 mice by oral administration of AZ-9, a bacterial polysaccharide from Klebsiella oxytoca

Ryosuke Sugihara, Masayasu Yoshimura, Masaharu Mori, Naoki Kanayama, Masaki Hikida, Hitoshi Ohmori

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Abstract

Collagen-induced arthritis (CIA) is an excellent model of rheumatoid arthritis (RA) in humans that is induced in DBA/1 mice immunized with bovine type II collagen (CII). Here, we report that the induction of CIA was effectively suppressed by oral administration of AZ-9, a purified polysaccharide with the average molecular weight of approximately 200 kDa that was produced by a soil bacterium, Klebsiella oxytoca. When AZ-9 was administered at 125-250 mg/kg/day orally for 9 consecutive days after immunization with CII followed by its administration every 3 days, resulted in a marked reduction of the incidence and the severity of CIA. The serum level of anti-CII IgG2a and the production of IFN-γ and IL-12 in the draining lymph node (LN) cells were significantly lower in AZ-9-administered mice than the untreated control. These findings suggest that orally administered AZ-9 suppressed CIA through attenuating a Th1-type response to CII. AZ-9 could be fragmented into smaller molecules (3-4 kDa) without losing its suppressive activity. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)325-333
Number of pages9
JournalImmunopharmacology
Volume49
Issue number3
DOIs
Publication statusPublished - Oct 5 2000

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Keywords

  • Anti-rheumatic agent
  • Bacterial polysaccharide
  • Collagen-induced arthritis
  • Th1 cytokines

ASJC Scopus subject areas

  • Pharmacology

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