Abstract
Collagen-induced arthritis (CIA) is an excellent model of rheumatoid arthritis (RA) in humans that is induced in DBA/1 mice immunized with bovine type II collagen (CII). Here, we report that the induction of CIA was effectively suppressed by oral administration of AZ-9, a purified polysaccharide with the average molecular weight of approximately 200 kDa that was produced by a soil bacterium, Klebsiella oxytoca. When AZ-9 was administered at 125-250 mg/kg/day orally for 9 consecutive days after immunization with CII followed by its administration every 3 days, resulted in a marked reduction of the incidence and the severity of CIA. The serum level of anti-CII IgG2a and the production of IFN-γ and IL-12 in the draining lymph node (LN) cells were significantly lower in AZ-9-administered mice than the untreated control. These findings suggest that orally administered AZ-9 suppressed CIA through attenuating a Th1-type response to CII. AZ-9 could be fragmented into smaller molecules (3-4 kDa) without losing its suppressive activity. (C) 2000 Elsevier Science B.V.
Original language | English |
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Pages (from-to) | 325-333 |
Number of pages | 9 |
Journal | Immunopharmacology |
Volume | 49 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2000 |
Keywords
- Anti-rheumatic agent
- Bacterial polysaccharide
- Collagen-induced arthritis
- Th1 cytokines
ASJC Scopus subject areas
- Pharmacology