Abstract
Because of a critical shortage in suitable organs, many patients with terminal liver disease die each year before liver transplantation can be performed. Transplantation of isolated hepatocytes has been proposed for the temporary metabolic support of patients awaiting liver transplantation or spontaneous reversion of their liver disease. A major limitation of this form of therapy is the present inability to isolate an adequate number of transplantable hepatocytes. A highly differentiated cell line, NKNT-3, was generated by retroviral transfer in normal primary adult human hepatocytes of an immortalizing gene that can be subsequently and completely excised by Cre/Lox site-specific recombination. When transplanted into the spleen of rats under transient immunosuppression, reversibly immortalized NKNT-3 cells provided life-saving metabolic support during acute liver failure induced by 90% hepatectomy.
| Original language | English |
|---|---|
| Pages (from-to) | 1258-1262 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 287 |
| Issue number | 5456 |
| DOIs | |
| Publication status | Published - Feb 18 2000 |
| Externally published | Yes |
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Cite this
Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. / Kobayashi, Naoya; Fujiwara, Toshiyoshi; Westerman, Karen A.; Inoue, Yusuke; Sakaguchi, Masakiyo; Noguchi, Hirofumi; Miyazaki, Masahiro; Cai, Jin; Tanaka, Noriaki; Fox, Ira J.; Leboulch, Philippe.
In: Science, Vol. 287, No. 5456, 18.02.2000, p. 1258-1262.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes
AU - Kobayashi, Naoya
AU - Fujiwara, Toshiyoshi
AU - Westerman, Karen A.
AU - Inoue, Yusuke
AU - Sakaguchi, Masakiyo
AU - Noguchi, Hirofumi
AU - Miyazaki, Masahiro
AU - Cai, Jin
AU - Tanaka, Noriaki
AU - Fox, Ira J.
AU - Leboulch, Philippe
PY - 2000/2/18
Y1 - 2000/2/18
N2 - Because of a critical shortage in suitable organs, many patients with terminal liver disease die each year before liver transplantation can be performed. Transplantation of isolated hepatocytes has been proposed for the temporary metabolic support of patients awaiting liver transplantation or spontaneous reversion of their liver disease. A major limitation of this form of therapy is the present inability to isolate an adequate number of transplantable hepatocytes. A highly differentiated cell line, NKNT-3, was generated by retroviral transfer in normal primary adult human hepatocytes of an immortalizing gene that can be subsequently and completely excised by Cre/Lox site-specific recombination. When transplanted into the spleen of rats under transient immunosuppression, reversibly immortalized NKNT-3 cells provided life-saving metabolic support during acute liver failure induced by 90% hepatectomy.
AB - Because of a critical shortage in suitable organs, many patients with terminal liver disease die each year before liver transplantation can be performed. Transplantation of isolated hepatocytes has been proposed for the temporary metabolic support of patients awaiting liver transplantation or spontaneous reversion of their liver disease. A major limitation of this form of therapy is the present inability to isolate an adequate number of transplantable hepatocytes. A highly differentiated cell line, NKNT-3, was generated by retroviral transfer in normal primary adult human hepatocytes of an immortalizing gene that can be subsequently and completely excised by Cre/Lox site-specific recombination. When transplanted into the spleen of rats under transient immunosuppression, reversibly immortalized NKNT-3 cells provided life-saving metabolic support during acute liver failure induced by 90% hepatectomy.
UR - http://www.scopus.com/inward/record.url?scp=0034681522&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034681522&partnerID=8YFLogxK
U2 - 10.1126/science.287.5456.1258
DO - 10.1126/science.287.5456.1258
M3 - Article
C2 - 10678831
AN - SCOPUS:0034681522
VL - 287
SP - 1258
EP - 1262
JO - Science
JF - Science
SN - 0036-8075
IS - 5456
ER -