Abstract
The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5-3mm diameter). When cumulus-oocyte complexes (COCs) from medium-sized follicles (MF; 3-6mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200ngmL-1 VEGF during the first 20h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200ngmL-1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200ngmL-1 during the first 20h of IVM.
| Original language | English |
|---|---|
| Pages (from-to) | 1902-1909 |
| Number of pages | 8 |
| Journal | Reproduction, Fertility and Development |
| Volume | 29 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2017 |
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Keywords
- pig
ASJC Scopus subject areas
- Biotechnology
- Reproductive Medicine
- Molecular Biology
- Animal Science and Zoology
- Genetics
- Endocrinology
- Developmental Biology
Cite this
Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles. / Bui, Tra M.T.; Nguyn, Khánh X.; Karata, Asako; Ferré, Pilar; Trn, Minh T.; Wakai, Takuya; Funahashi, Hiroaki.
In: Reproduction, Fertility and Development, Vol. 29, No. 10, 2017, p. 1902-1909.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles
AU - Bui, Tra M.T.
AU - Nguyn, Khánh X.
AU - Karata, Asako
AU - Ferré, Pilar
AU - Trn, Minh T.
AU - Wakai, Takuya
AU - Funahashi, Hiroaki
PY - 2017
Y1 - 2017
N2 - The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5-3mm diameter). When cumulus-oocyte complexes (COCs) from medium-sized follicles (MF; 3-6mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200ngmL-1 VEGF during the first 20h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200ngmL-1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200ngmL-1 during the first 20h of IVM.
AB - The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5-3mm diameter). When cumulus-oocyte complexes (COCs) from medium-sized follicles (MF; 3-6mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200ngmL-1 VEGF during the first 20h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200ngmL-1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200ngmL-1 during the first 20h of IVM.
KW - pig
UR - http://www.scopus.com/inward/record.url?scp=85029031146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029031146&partnerID=8YFLogxK
U2 - 10.1071/RD16321
DO - 10.1071/RD16321
M3 - Article
C2 - 27938625
AN - SCOPUS:85029031146
VL - 29
SP - 1902
EP - 1909
JO - Reproduction, Fertility and Development
JF - Reproduction, Fertility and Development
SN - 1031-3613
IS - 10
ER -