Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes

Hiroaki Funahashi, Nam Hyung Kim, Todd T. Stumpf, Thomas C. Cantley, Billy N. Day

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The effects of organic osmolytes on cytoplasmic maturation of porcine oocytes were examined in maturation medium (modified Whitten's medium) containing various NaCl concentrations. The presence of organic osmolytes, such as taurine and sorbitol, at 6 and 12 mM in maturation medium containing 68.49 or 92.40 mM NaCl increased oocyte glutathione content. Microfilament organization in oocytes was disrupted in maturation medium containing the higher level of NaCl (92.40 mM). However, supplementation with 12 mM sorbitol to the medium reduced the severity of the abnormality. Early embryonic development in vitro to the blastocyst stage was 8.3 ± 0.9% for oocytes matured in modified Whitten's medium (68.49 mM NaCl) supplemented with 12 mM sorbitol, and 7.9 ± 0.8% in modified NCSU23 medium (containing 108.73 mM NaCl, 7 mM taurine, 9 mM hypotaurine, and 1 mM glutamine), compared to 4.7 ± 0.6% in modified Whitten's medium (68.49 mM NaCl), which did not contain organic osmolytes. These results indicate that the presence of organic osmolytes, such as sorbitol and taurine, reduces the detrimental effects of high NaCl concentration in media used for the maturation of porcine oocytes. This effect is reflected by oocyte glutathione content and microfilament organization at the end of maturation and early development following in vitro maturation and in vitro fertilization.

Original languageEnglish
Pages (from-to)1412-1419
Number of pages8
JournalBiology of Reproduction
Volume54
Issue number6
Publication statusPublished - Jun 1996
Externally publishedYes

Fingerprint

Oocytes
Swine
Sorbitol
Taurine
Actin Cytoskeleton
Glutathione
Blastocyst
Fertilization in Vitro
Glutamine
Embryonic Development
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. / Funahashi, Hiroaki; Kim, Nam Hyung; Stumpf, Todd T.; Cantley, Thomas C.; Day, Billy N.

In: Biology of Reproduction, Vol. 54, No. 6, 06.1996, p. 1412-1419.

Research output: Contribution to journalArticle

Funahashi, Hiroaki ; Kim, Nam Hyung ; Stumpf, Todd T. ; Cantley, Thomas C. ; Day, Billy N. / Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. In: Biology of Reproduction. 1996 ; Vol. 54, No. 6. pp. 1412-1419.
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