Presence of macrophage migration inhibitory factor (MIF) in ependyma, astrocytes and neurons in the bovine brain

Masahiro Nishibori; Naoki Nakaya; Akihito Tahara; Masahiro Kawabata; Shuji Mori; Kiyomi Saeki

doi:10.1016/0304-3940(96)12864-0

Presence of macrophage migration inhibitory factor (MIF) in ependyma, astrocytes and neurons in the bovine brain

Masahiro Nishibori, Naoki Nakaya, Akihito Tahara, Masahiro Kawabata, Shuji Mori, Kiyomi Saeki

Research output: Contribution to journal › Article › peer-review

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Abstract

We investigated the immunohistochemical localization of a cytokine macrophage migration inhibitory factor (MIF) in the bovine brain. MIF was present in the ependymal cell linings of the cerebral ventricles throughout. Double immunostaining of the section with anti-glial fibrillary acidic protein (GFAP) antibody and with anti-MIF antibody showed that the astrocytes present in subependymal layer were immunoreactive for MIF. In the hippocampus, the pyramidal cells in the CA3 and CA4 subfields and the granule cells of the dentate gyms were immunoreactive. The bundles of messy fibers were stained along their projections to CA3 and CA4 regions. The nuclei of the subpopulation of these MIF-immunoreactive cells were also immunostained. These results indicated the widespread distribution of a cytokine, MIF, in the bovine brain and suggested the possibility that MIF might play additional roles than a proinflammatory mediator role in the brain.

Original language	English
Pages (from-to)	193-196
Number of pages	4
Journal	Neuroscience Letters
Volume	213
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(96)12864-0
Publication status	Published - Aug 9 1996

Keywords

Astrocytes
Bovine brain
Ependyma
Macrophage migration inhibitory factor
Neurons

ASJC Scopus subject areas

Neuroscience(all)

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title = "Presence of macrophage migration inhibitory factor (MIF) in ependyma, astrocytes and neurons in the bovine brain",

abstract = "We investigated the immunohistochemical localization of a cytokine macrophage migration inhibitory factor (MIF) in the bovine brain. MIF was present in the ependymal cell linings of the cerebral ventricles throughout. Double immunostaining of the section with anti-glial fibrillary acidic protein (GFAP) antibody and with anti-MIF antibody showed that the astrocytes present in subependymal layer were immunoreactive for MIF. In the hippocampus, the pyramidal cells in the CA3 and CA4 subfields and the granule cells of the dentate gyms were immunoreactive. The bundles of messy fibers were stained along their projections to CA3 and CA4 regions. The nuclei of the subpopulation of these MIF-immunoreactive cells were also immunostained. These results indicated the widespread distribution of a cytokine, MIF, in the bovine brain and suggested the possibility that MIF might play additional roles than a proinflammatory mediator role in the brain.",

keywords = "Astrocytes, Bovine brain, Ependyma, Macrophage migration inhibitory factor, Neurons",

author = "Masahiro Nishibori and Naoki Nakaya and Akihito Tahara and Masahiro Kawabata and Shuji Mori and Kiyomi Saeki",

note = "Funding Information: We gratefullya cknowledgSeh izukoYokoyama for her excellent technical assistance,T his work was partly supported by Grant-in-Aid No. 06670114 from the Japanese Ministry of Education, Science, Sports and Culture, Japan and a Grant from Ryobiteien Memorial Foundation.",

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doi = "10.1016/0304-3940(96)12864-0",

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AU - Nishibori, Masahiro

AU - Nakaya, Naoki

AU - Tahara, Akihito

AU - Kawabata, Masahiro

AU - Mori, Shuji

AU - Saeki, Kiyomi

N1 - Funding Information: We gratefullya cknowledgSeh izukoYokoyama for her excellent technical assistance,T his work was partly supported by Grant-in-Aid No. 06670114 from the Japanese Ministry of Education, Science, Sports and Culture, Japan and a Grant from Ryobiteien Memorial Foundation.

PY - 1996/8/9

Y1 - 1996/8/9

N2 - We investigated the immunohistochemical localization of a cytokine macrophage migration inhibitory factor (MIF) in the bovine brain. MIF was present in the ependymal cell linings of the cerebral ventricles throughout. Double immunostaining of the section with anti-glial fibrillary acidic protein (GFAP) antibody and with anti-MIF antibody showed that the astrocytes present in subependymal layer were immunoreactive for MIF. In the hippocampus, the pyramidal cells in the CA3 and CA4 subfields and the granule cells of the dentate gyms were immunoreactive. The bundles of messy fibers were stained along their projections to CA3 and CA4 regions. The nuclei of the subpopulation of these MIF-immunoreactive cells were also immunostained. These results indicated the widespread distribution of a cytokine, MIF, in the bovine brain and suggested the possibility that MIF might play additional roles than a proinflammatory mediator role in the brain.

AB - We investigated the immunohistochemical localization of a cytokine macrophage migration inhibitory factor (MIF) in the bovine brain. MIF was present in the ependymal cell linings of the cerebral ventricles throughout. Double immunostaining of the section with anti-glial fibrillary acidic protein (GFAP) antibody and with anti-MIF antibody showed that the astrocytes present in subependymal layer were immunoreactive for MIF. In the hippocampus, the pyramidal cells in the CA3 and CA4 subfields and the granule cells of the dentate gyms were immunoreactive. The bundles of messy fibers were stained along their projections to CA3 and CA4 regions. The nuclei of the subpopulation of these MIF-immunoreactive cells were also immunostained. These results indicated the widespread distribution of a cytokine, MIF, in the bovine brain and suggested the possibility that MIF might play additional roles than a proinflammatory mediator role in the brain.

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KW - Ependyma

KW - Macrophage migration inhibitory factor

KW - Neurons

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Presence of macrophage migration inhibitory factor (MIF) in ependyma, astrocytes and neurons in the bovine brain

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